Bacterial blight disease caused by Xanthomonas axonopodis pv. manihotis (Berthet-Bondar) Dye was assessed in 11 artificially inoculated cassava genotypes in a screenhouse. Disease progress was estimated at intervals of 3 days by measuring the length of necrotic lesions on stems and leaves, as well as estimating the average disease score and area under disease progress curve (AUDPC). Based on the average disease scores, cassava genotypes 30572, TME 1, TME 7 and TME 9 were classified as resistant to bacterial blight, genotypes 4(2)1425, TME 2, TME 4 and TME 12 were tolerant while cassava genotypes 30001, TME 3, and TME 28 were susceptible. Direct correlations, statistically significant at p 5 0.05, were obtained between stem necrosis, leaf necrosis, average disease scores and AUDPC in the 11 cassava genotypes. Screenhouse experiments afford rapid assessment of resistance status of cassava genotypes to bacterial blight in Nigeria.

No abstract

Bacteria identification in fish is important; hence it provides update information on emerging and existing organisms thereby enriching the gene bank on fish disease. This study investigated bacteria associated with milt in cultured samples of African catfish- Clarias gariepinus. Catfish broodstock were collected from private Fish Farm in Ogun State, Nigeria. Fish samples were taken to the Microbiology laboratory, Federal University of Agriculture, Abeokuta Ogun State for milt microbial analyses, and amplicon were taken to International Institute of Tropical Agriculture, Ibadan for molecular characterization. Bio-edit was use for importing and mining nucleotide sequences into gene bank. The results revealed that the following bacterial organisms were present in the milt of Clarias gariepinus: Aeromonas caviae, Proteus mirabilis, Serratia rubidaea, Pseudomonas mosselii, Acinetobacter soli and Klebsiella variicola. The Basic Local Alignment Search Tools revealed the percentage similarity ranging from 86- 97.04% and their accession numbers. These bacteria indicated high levels of faecal contamination in the environment. In conclusion, bacteria were found in the milt of cultured catfish and are capable of being pathogenic to humans and may increase the vertical transfer to fry during breeding and rearing stage.

No abstract