The spring wheat variety Varyag whith high potential nitrogenase activity and the greater contents of chlorophyll in leaves and the active strain of the bacteria Azospirillum sp. 77, selected from the washed roots of the spring wheat variety Varyag, were used for creation of effective association “wheat – diasotrophs of genus Azospirillum”. Inoculation of wheat variety Varyag seeds with the bacterial suspension Azospirillum sp. 77 promoted the reliable increase of nitrogenase activity on roots of plants (by 46 %), content of chlorophyll in leaves (by 18 %) and structural parameters of wheat crop (weight of grain from 1 ear and weight of 1000 grains were increased as a result of inoculation by 16,5 and 16 % accordingly).