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An ozonation-biological filtration pilot-scale study was performed to evaluate the formation and removal of biodegradable ozonation by-products. The formation of aldehydes and ketoacids was found to be proportional to the DOC concentration and ozone dosage, and a strong relationship between the formation of aldehydes, ketoacids, and biodegradable dissolved organic carbon (BDOC) was observed. Four types of granular activated carbon (GAC) and one nonadsorbing medium, biolite, were employed to evaluate the performance of biofiltration for removing ozonation by-products. It was observed that GAC filters developed biological activity sooner than the biolite filter. Once developed, biofilters, either GACs or biolite, were particularly effective in the removal of aldehydes, ketoacids and BDOC.