Three yeast strains, Kluyveromyces marximanus NRRL-3234 (KM), Saccharomyces cerevisiae NCDC-42 (SC) and Saccharomyces uvarum ATCC-9080 (SU), and a mixed culture (1:1:1 ratio) were evaluated for their value as probiotics in lamb feeding in two experiment. In experiment I and II, 20 and 30 pre-weaner lambs were fed for 63 and 60 days in two and three equal groups respectively. All lambs were offered ad libitum a creep mixture and Zizyphus nummularia leaves, and yeasts were dosed orally. In experiment I, one group received no yeast, the other of the mixed culture (1.5-2 x 10(10) live cells/ml). In experiment II, yeast cultivation was modified yielding 1.5-2 x 10(13) live cells/ml. Lambs of the three experimental groups received 1 ml/kg live weight of one of the individual yeasts. Feed intake did not differ among groups of both experiments with the exception of SC-supplemented lambs in experiment II which showed a trend to higher intakes per kg metabolic body weight and in percentage of body weight when compared with KM- and SU-supplemented lambs. Supplementation of the mixed yeast culture had no effect on intakes of digestible crude protein and metabolisable energy, nutrient digestibility, nitrogen balance and rumen fermentation characteristics (pH, ammonia, volatile fatty acid concentration, protozoa count) and urinary allantoin as an indicator of microbial protein synthesis. The same was true for comparisons in experiment II except ciliate protozoa counts, which showed a trend to be the highest with SU and the lowest with SC. The results of present study show that the response of lambs to supplemented live yeast cultures is inconsistent, as it lacked to have an effect in the present study, and that differences among strains were small, even when supplemented at a much higher live cell count.
Malpura and Kheri ewes (76) in their late gestation, weighing 34.40±0.95 kg were randomly selected and divided into 4 groups of 19 each (G1, G2, G3 and G4). Ewes in all the groups were grazed on natural rangeland from 07.00 h to 18.00 h. Ewes in G1were maintained on sole grazing while ewes in G2, G3 and G4, in addition to grazing received concentrate mixture at the rate of 1% of their body weight during late gestation, early lactation and entire last quarter of pregnancy to early quarter of lactation, respectively. The herbage yield of the community rangeland was 0.82 metric ton dry matter/hectare. The diet consisted of (%) Guar (Cyamopsis tetragonoloba) bhusa, (59.2), Babool pods and leaves (17.2), Bajra (Pennisetum typhoides) stubbles (8.8), Doob (5.3), Aak (4.2) and others (5.3). The nutrient intake and its digestibility were higher (p<0.01) in G2, G3 and G4 as compared to G1 because of concentrate supplementation. The intakes of DM (g/kg W 0.75 ), DCP (g/kg W 0.75 ) and ME (MJ/kg W 0.
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