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An expert system for the interpretation of infrared spectra EXPIRS was created. The main features of EXPIRS are: hierarchical organization of the characteristic groups, realized by frames; registration of the multiple use of spectral bands; taking into account the solvent absorption and the chemical inconsistencies; documenting the interpretation course and providing explanations on request. The ten most important heuristics used by an expert for interpretation of infrared spectra were formulated and some of them were tested with EXPIRS.

The effects of a new intestinal peptide, inhibiting enterocytogenin (IEG) derived from pig intestinal mucosa were studied in vitro on 3T3 mouse fibroblasts and L5178Y mouse lymphoma cell line. IEG caused considerable growth inhibition together with specific morphological changes, necrotic effects as well as formation of monolayers at the highest concentration applied (1000 micrograms/ml). A biologically active fraction (IEG-BAF) derived by further purification of IEG by gel-filtration, proved to possess most of the described activity. The concentrations of IEG and IEG-BAF inhibiting the growth of L5178Y lymphoma cells by 50% (IC50 values) were calculated to be 759 micrograms/ml and 192 micrograms/ml, respectively. IEG-BAF has a molecular mass of 4450 +/- 180 Da and is most probably a peptidylnucleotidate as revealed by spectral analysis.