This study was aimed to show the effect of adding different levels of grape pomace (GP) and resveratrol (RSV) to the diet of laying hens on production performance and antioxidant capacity. Seventy two laying hens (Lohmann Brown) were used to study cumulative production performance and antioxidant activites. The experimental diets were; the first treatment T1 (control), T2 (1% GP), T3 (0.5% GP), T4 (400 mg/kg RSV), T5 contained on high-energy, low-protein diet (HELP) without any additives. T6, (HELP + 1% GP), T7, (HELP + 0.5% GP) and T8 (HELP + 400 mg/kg RSV). The results showed that There were no significant differences in the type of diet in the concentration of glucose, cholesterol and HDL, while a significant decrease (P≤0.01) in cholesterol concentration was observed in favor of the addition treatments (grape pomace 1%), (grape pomace 0.5%) and (resveratrol). 400 mg/kg feed) compared to the treatment (without addition), the results of the study showed a significant decrease (P≤0.01) in the gene expression of the SOD gene in the addition and interference treatments in all experiment treatments compared to the control treatment. The results of the study also indicated a high Significant (P≤0.05) in IL-6 gene expression in favor of T8 treatment compared to the control treatment. In conclusion, differences in grape pomace and resveratrol levels demonstrate the unique ability of natural antioxidants to improve physiological traits and gene expression for the prevention of hemorrhagic fatty liver syndrome in laying hens.

This study was conducted at the local hatchery in Ramadi, Anbar Iraq from 1st to 23th Dec. 2018, to 26 Feb 2019. The objectives of this study were to investigate the effect of injected eggs hatching in times and different concentrations of Biotin in growth and embryonic development, hatchability. Six hundred eggs of hatching types (Ross 308) and injected with different concentrations of biotin at age of 0 days (before placing in the hatchery) and 18 days of incubation. Eggs were divided into five groups (120 eggs for each) as follows: 1.T0: Control group placed in the hatchery without injection. 2.T1: Injected with a dose of 100 μg biotin at age of zero. 3. T2: Injected with dose 75 μg biotin at age zero. 4.T3: Injected with a dose of 100 μg biotin at age 18 days of incubation. 5.T4: Injected with a dose of 4175 μg biotin at age of 18 days of incubation. Statistical analysis was performed (CRD) (P=0.065) results show: Increase length of the embryo, diameter of vascular region and number of pairs of somites at 3 days of incubation for T2. Increase percentage of embryonic weight, decrease the percentage of albumin and the percentage of at 7 days of incubation for T1 and T2. Increase in percentage of embryonic weight and amniotic sac and liquid, decrease in the percentage of albumin and yolk, at 14 days of incubation for T2. Increase percentage of embryonic weight, decrease the percentage of yolk at the age of 17 days incubation for T2. Increase hatchability of total eggs and fertile eggs. Decrease embryonic mortality and different stages of embryonic development. Concluded that In-Ovo injection of the hatching eggs with biotin contributed to increase physiological traits and embryonic development.