In this study, the effects of various kinds of sawdust and supplements on the yield of Ganoderma lucidum were investigated in artificial cultivation. The sawdust of Alnus nepalensis, Shorea robusta and Dalbergia sisoo and supplements of rice bran , wheat bran, corn flour and gram flour were used as substrates in Ganoderma lucidum cultivation . Sawdust alone was used in control as a substrate media in the cultivation. Significant differences (P< 0.05) were found among varieties of sawdusts and supplements. Similarly significant differences (P<0.05) were found in sawdust with supplements and without supplements in yield and biological efficiency. Dalbergia sisoo sawdust could not give yield in ambient condition; Shorea robusta gave very low yield and biological efficiency. Alnus nepalensis gave good yield and biological efficiency compared to the other sawdust.The highest yield and Biological efficiency were obtained from gram flour compared to the other brans. Alnus nepalensis sawdust supplemented with gram flour showed higher yield among all treatments. Supplementation showed positive role in mycelia growth and yield of mushroom.