Agroforestry systems (AFSs) that have rubber tree as the main tree component are important productive systems in the Brazilian Amazon. This work aimed to evaluate carbon stock (CS) of tree biomass, litter and soil in rubber based AFSs in South-Western Amazon. The analytical observational study was carried out in Rolim de Moura (RO) from June 2018 to March 2019 and it consisted of five areas: three AFSs, a forest fragment (FL), and a pasture (PA). CS of tree biomass was determined using allometric equations, while litter and soil carbon stocks were performed through material collection and chemical analysis.