Time-of-flight MALDI mass spectrometry (MALDI-TOF-MS) profiling of blood serum of patients with Guillain-Barré syndrome (GBS, 36 samples), chronic inflammatory demyelinating polyneuropathy (CIDP, 24 samples) and practically healthy donors (HD) (35 samples) was carried out in order to identify potential biomarkers of autoimmune demyelinating polyneuropathies (ADP). To simplify the peptide-protein mixture of serum prior to MALDI-TOF-MS analysis samples were pre-fractionated on magnetic microparticles with a weak cation-exchange (MB-WCX) surface. Comparative analysis of mass spectrometric data using the classification algorithms (genetic and neural network-controlled) revealed a characteristic set of peaks, agreed change area with a high specificity and sensitivity of the differentiated mass spectrometry profiles of the blood serum of patients with DPNP and healthy donors (for GBS values of these characteristics reached 100 and 100, and for CIDP 94.1 and 100% respectively). Comparative analysis of mass spectrometric profiles of serum samples obtained from patients with GBS and CIDP, allowed to build a classification model to differentiate these diseases from each other, with a specificity of 88.9 and a sensitivity of 80%.

A clinical case of a combined course of Epstein-Barr viral infection and mumps in a 20-year-old man is presented. The combination of clinical symptoms characteristic of Epstein-Barr viral infection and hypersalivation, followed by an increase in α-amylase in the blood, which developed a few days after hospitalization, caused the extension of the diagnostic search in order to clarify the diagnosis. Diagnostic errors and difficulties encountered in the management of patients with upper respiratory tract lesions were noted.Funding. The study had no sponsor support. Conflict of interest. The authors declare no conflict of interest.

Федеральное государственное бюджетное образовательное учреждение высшего образования «Московский государственный медикостоматологический университет имени А.И. Евдокимова» Министерства здравоохранения Российской Федерации, 127473, г. Москва, Российская Федерация 2 Государственное бюджетное учреждение здравоохранения «Инфекционная клиническая больница № 2» Департамента здравоохранения г. Москвы,