Dynamics of oxidation of inorganic sulphur compounds to sulphate by the soil of spruce forests was investigated. Sulphide, sulphite and thiosulphate are oxidized to sulphate at a maximal rate at the beginning of the reaction, oxidation of elemental sulphur exhibits a lag phase. Linear relationships between the amounts of the produced sulphate and concentrations of substrates in the soil could be detected. On the basis of ;this finding a method for comparison of the oxidative activity of various soils was proposed.

Some factors influencing the oxidative activity of upper horizons of spruce forest soils (a mixture of fermentative and humus layers) toward intermediates of the oxidative part of the sulphur cycle were investigated. Preincubation of the soil with added cysteine, sulphide, elemental sulphur or thiosulphate was found to stimulate enzyme systems oxidating any of these compounds. Sulphite and sulphate were ineffective in this respect. The oxidation of elemental sulphur was stimulated by CaCO3, technical urea and high doses of superphosphate and potassium sulphate. It was inhibited by KH2PO4, pure urea, 40 % potassium salt, ammonium nitrate with calcium carbonate and the fertilizer NPK I. It proceeded at the highest rate at approximately 60 % capillary capacity (61 % of mass water content). Oxidation of thiosulphate was stimulated by KH2PO4, pure urea, superphosphate, potassium sulphate and only slightly by the fertilizer NPK I. It was inhibited by CaCO3, 40 % potassium salt and only slightly by ammonium nitrate with calcium carbonate. Potassium chloride, glucose and technical urea were without effect. The oxidation proceeded at the highest rate at 35 % maximal capillary capacity (48 % mass water content).

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Effect of industrial emissions with high sulphur dioxide content on the upper horizons of spruce forest soils in NW Bohemia was investigated. The content of sulphates, oxidative activity towards sulphide, elemental sulphur, thiosulphate and sulphite, concentration and species representation of thiobacilli in horizons F, H and A in regions highly affected by emissions (two localities) and in regions relatively less influenced (three localities) were followed. In the affected areas the sulphur content in the soil was higher, the species representation of thiobacilli was similar and their concentration was higher, the ability of the soil to oxidize thiosulphate was inhibited and oxidation of elemental sulphur was stimulated. The oxidation of sulphide and sulphite was not significantly affected by the emissions. Changes caused by emissions could be observed only in horizons F and H and did not involve horizons A.

The species representation of Thiobacilli was investigated in horizons F, H and A of spruce forest at ten localities. Concentrations of Thiobacilli in the selected localities and ability of the soils to oxidize sulphur compounds to sulphate were determined. Horizons F exhibited a high oxidative activity, a lower activity was found in horizon H and the lowest one was detected in horizon A. The activities showed spring and autumn maxima. Horizons F, H and A contained 10(4)--10(5), 10(2)--10(3) and 10(1)--10(3), respectively, Thiobacilli in 1 g dry soil. Thiobacillus thioparus was detected in all three horizons from all localities, T.thiooxidans was found in all horizons F, only in some horizons H and was not detected in horizons A. T.novellus was found only in some samples without any relation to the horizons, T.denitrificans was not detected at all.