The basic parameters of hydrogel membranes, which are important for their characteristics as potential biocompatible non-degradable wound coverings, have been optimized. Moderately hydrophobic and at the same time moderately hydrophilic membranes obtained on the basis of copolymerization of acrylamide and acrylonitrile were demonstrated to be biocompatible with mesenchymal stem cells. Small concentrations of highly dispersed silica introduced into a composition of hydrogel membranes were demonstrated to influence the cell cultivation positively. The technological conditions necessary for more effective and prolonged cultivation of cells on the surface of hydrogel membranes with improved exploitation parameters have been established.

The In sti tute of Biocolloid Chem is try named af ter F.D. Ovcharenko, NAS of Ukraine 42, Ac a de mi cian Vernadsky blvd., Kyiv, 03142, Ukraine 1 The In sti tute of Mo lec u lar Bi ol ogy and Ge net ics, NAS of Ukraine 150, Ac a de mi cian Zabolotny Str., Kyiv, 03143, Ukraine Sig nif i cant amount of hydrogel mem branes for stem cells cul ti va tion has been syn the sized on the ba sis of acrylamide, acrylonitrile, and acrylic acid by the method of rad i cal copolymerization. The com par a tive in ves ti ga tions have shown that the op ti mal pa ram e ters, for the im mo bi li za tion of hu man mesenchymal stem cells are in her ent to nonionic co pol y mer hydrogels on acrylamide and acrylonitrile bases. The use of hydrogels with con sid er able acrylic acid con tent is lim ited by pH de crease in cul ti va tion me dium, which is in com pat i ble with life ac tiv ity of the cells un der in ves ti ga tion.