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Objectives A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea. To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro. Methods The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC–MS). Nitric oxide (NO) assay was determined using the Griess reaction. Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry. Arginase activity was examined by colorimetric method. Results The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release. Conclusions Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.

Aim. The aim of the investigation was to study the effect of lipopolysaccharides (LPS) derived from saprophytic strains of Pseudomonas aeruginosa on the resistance to phytopathogenic strain of P. aeruginosa IMB 9096. The wild-type (Col-0) Arabidopsis thaliana plants, npr1 mutant, which lacks expression of PR-genes, NahG genotype plants, expressing the bacterial gene of NahG salicylate hydrolase, jin1 mutant, insensitive to jasmonic acid, have been used as a model systems in resistance testing. Methods. Common phytopathological methods were used. Results. Lipopolysaccharide from the saprophyte P. aeruginosa IMV 8614 strain increased the resistance of seedlings of all genotypes to infection with phytopathogenic strain P. aeruginosa IMB 9096. The most effective protection had been observed in the mutant jin1. The protective effect was also observed in jin1 after the treatment with LPS derived from the saprophyte strain P. aeruginosa IMV 8615. LPS 8615 increased the sensitivity to infection in the NahG and npr1 transgenic plants, especially in NahG. LPS from the saprophyte P. aeruginosa IMV 8616 increased resistance to P. aeruginosa IMB 9096 infection in all four A. thaliana genotypes. Conclusions. The effect of LPS derived from different strains of saprophytic bacteria can both increase and decrease the sensitivity of plants to infection with bacterial phytopathogens. The effect of LPS depends upon the bacteria strain and the functional state of the salicylate and jasmonate signaling systems in the infected plants.Keywords: Arabidopsis thaliana, Pseudomonas aeruginosa, lipopolysaccharide, induced resistanse