This study describes the application of electrospray ionization mass spectrometry in order to identify ten antibiotics (macrolides, penicillins, aminoglycosides). An optimum procedure was developed for determination of antibiotics of dierent grade. Positive ion spectra of most antibiotics are higher in intensity including an [M+H] + ion and produce less fragmentation and are more informative compared to the negative ion spectra. The group of antibiotics exhibits the same characteristic fragmentation. The data base was developed for identication of antibiotics comparing of their molecular and fragment ions. The results of the study showed that the method with electrospray ionization is simple and quick which is useful in the routine determination of antibiotics and in their pharmaceutical dosage forms.

The aim of the paper is the identification of the most frequently used antibiotics such as cefazolin, cefuroxime, cefotaxime, ceftriaxone, ceftazidime, lyncomycin, clindamycin, levofloxacin, doxycycline and vancomycin by electrospray ionization mass spectrometry interface in positive and negative modes. The fragmentation pathways of the group of antibiotics were colligated on the basis of the obtained ESI mass spectra. Generally, the positive ion spectra of antibiotics are higher in intensity including an [M+H] + ion and producing less fragmentation as well as more informative compared to the negative ion spectra. Only ESI of levofloxacin in positive and negative modes with the chosen tune parameters leads to degradation of molecular ion, so the parent ion does not appear in the mass spectra. The group of antibiotics (cephalosporins and lincosamides) shows the same characteristic fragmentation. These setup parameters were successfully applied for the routine determination of antibiotics in their pharmaceutical dosage forms and other objects. Data base was developed for identification of antibiotics by comparison of their molecular and fragment ions.

In the paper, two-stage signal pre-processing algorithm based on the ltration is presented. The developed algorithm is dedicated for the diagnostic programmable device PUD-2. The PUD-2 is the real-time analyzer based on programmable logic devices FPGA, as well as on ARM processor. Application of FPGA programmable devices and ARM processors allows to merge advantages of hardware and software implementations. Further, analysis of digital lters parameters in case of its ecient realization on the FPGA is presented. The aim of the study is to select digital-lter parameters in such way that the available resources of FPGA are used eciently and lter characteristics meet established criteria. In the study, low pass nite impulse response and innite impulse response lters are compared. For the rst stage of the signal pre-processing algorithm, hardware implementation of the innite impulse response lter is proposed, contrary to the second stage, where software realization of the nite impulse response lter is suggested. Combination of hardware and software ltration algorithms allows for fast and ecient realization of signal pre-processing algorithm used in analysis carried out on the PUD-2.