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The study was conducted to evaluate the applicability of ultra high-performance liquid chromatography - tandem mass spectrometry method (UHPLC-MS/MS), establish the MS/MS detection parameters and determine the validation characteristics for the analysis of residual content of avermectins in milk. The UHPLC-MS/MS method has proved to be accurate, practical and universal. This was confirmed by Decision limit (CCα) data: abamectin - 12.56 μg/kg, doramectin - 17.74 μg/kg, eprinomectin - 24.02 μg/kg, ivermectin - 12.53 μg/kg, moxidectin - 44.69 μg/kg, recovery is 88.7-110%. The data obtained for assessing the suitability, accuracy and reproducibility of the results meet the requirements of the European Directive (2002/657/EC). The efficient ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method that was developed and adopted for routine use by the laboratories of veterinary medicine, allows to detect residual quantities of 5 avermectins used in animal breeding for the prevention of helminthiases in food products including milk.

A liquid chromatographic-tandem mass spectrometric (LC–MS/MS) multi-residue method has been used for the simultaneous quantification and identification of 9 residues and metabolites of benzimidazole derivatives that the most widely used as anthelmintic veterinary drugs of animals. The modified QuEChERS method was used for sample preparation, which was initially developed for pesticide residue analysis. This paper highlights how quick, easy, cheap, effective and rugged the QuEChERS extraction method is. The method was successfully validated according to the 2002/657/EC guidelines. Recovery of analytes was in the range 99 – 110 %. The decission limits (ССα ) were calculated at MRL level for analytes with an established permitted limit as next: 104.5 μg kg-1 (albendazole), 53.2 μg kg-1 (fenbendazole), 54.2 μg kg-1 (flubendazole), 12.1 μg kg-1 (levamisole), 53.9 μg kg-1 (cambendazole), 64.2 μg kg-1 (mebendazole), 52.5 μg kg-1 (parabendazole), 105.8 μg kg-1 (thiabendazole), 234.2 μg kg-1 (triclobendazole). The suitability of the assay has been assessed through InterVal Software by quo data GmbH (Germany). The method achieves high quality of the results, good recovery, repeatabilities, within-lab reproducibilities, and wide analytical scope and has practical benefits, low cost, high sample throughput, little labor used and few lab ware.