Purpose:In aquaculture, the most important aspect to consider is ensuring the development of valuable offspring by using high-quality gametes from fish broodstock. This research aims to compare the biochemical constituents of seminal plasma in both wild and cultured Clarias gariepinus broodstock.Research Method: Apparently healthy adult male Clarias gariepinus (n=9, 900±300g mean weight per fish type (wild or cultured), 49±8.5cm mean total length) were collected from fishermen on the Oshin River, Nigeria for the wild samples and from a reputable fish farm in Ilorin and examined for some seminal plasma's biochemical parameters following standard procedures. T-test statistics were used to compare the differences between classes. Findings: Higher values of most of the semen biochemical compositions were recorded among the cultured Clarias gariepinus with significant variations (p<0.05) recorded in the mean values of total cholesterol, high-density lipoprotein-cholesterol (HDL-Cholesterol), low-density lipoprotein-cholesterol (LDL-Cholesterol), uric acid, Aspartate Aminotransferase (AST), total bilirubin, albumin, potassium, chloride and urea. Creatinine, glucose, sodium, and triglycerides, on the other hand, did not vary significantly (p>0.05) between cultured and wild Clarias gariepinus semen. Originality/ Value:The current study's findings on biochemical compositions could lead to more effective gamete management, higher yields, and improved semen suitability for short-term storage.

Heterotis niloticus (average weight: 3.5 kg ± 101 g) were collected, acclimated for 14 days, euthanized, and stored at ambient temperature (27 ± 3°C) for 24 hr. Approximately 42 samples were collected at 0, 4, 8, 12, 16, 20, and 24 hr for sensory assessment, histology, biochemical, and microbiological analyses. Data were analyzed using appropriate statistical tool with SPSS 16.0 version. H. niloticus retained most of its original sensory attributes of freshness during the first 12‐hr post‐slaughter time interval (PSI). The histology shows that muscle tissues were intact, the connective tissues were not widely separated, and the biochemical and microbial results were within the acceptable limits. Most of the good physical features used to judge fish freshness were still retained till 12‐hr post‐slaughter time after which the sensory attributes of the fish fell below average. Therefore, H. niloticus should not be delayed beyond 12‐hr PSI. Novelty impact statement This study is a novel study detailing the effect of post‐slaughter time intervals on the sensory, histology, biochemical, and microbial qualities of Heterotis niloticus. The results of this study will guide dieticians, food processors, and quality assurance agencies to look out for the qualities of H. niloticus so as to solve the malnutrition problems in patients.