With the use of technology of artificial insemination in sheep farms are not yet defined criteria for assessing the full value of the preparation of females for insemination, and hence – the possibility of prediction and correction of their fertilization, which prevents the rational use of cryopreserved semen and ensuring the maximum reception of the offspring. We have proved the prognostic importance of fertilization of the morphofunctional state of the vulva and the vagina and the quality of mucus in sheep breeds during estrus. The material of the study was 327 sheep of ascanian breeding, which during the sexual intercourse before insemination examined the vulva, vagina and evaluated the estrus slime (number, color, consistency, presence of impurities, elasticity, electrical resistance, type of crystallization, protein content). The results of ultrasound diagnosis of pregnancy were determined by the fertility of sheep with different integral compositions of clinical and laboratory parameters. It has been established that in sheep, in which fertility in the first sexual cycle reached 65.1%, most often found a pink, moderately edematous vulva with clear mucus. In females with pale mucous membrane and insignificant amount of mucus, the effectiveness of inseminates declined to 53.3-58.0%. In a significant amount of estral mucus, the proportion of infertile animals increased twofold. At the same time, the selection of liquid, but cloudy, or thick mucus is a sign of an unfavorable prognosis, in which fertility decreases by 1.8-2.1 times (p <0.001). Dense, white, paste-like isolates were observed in a small number of sheep, mostly bright at the beginning of the anestral season. Low fertility in the first sexual hunting (35.5%) and a high multiplicity of repeated inseminations (29.0%) indicate that sheep with thick estral slime are only beginning to enter the sexual season, and this quality of secrecy indicates an inadequate estrogenization of the body. It was also found that in the infertile sheep during sexual hunting, the protein content of cervical mucus was 4.8 times higher, and the elasticity of mucus was reduced by 2.9 times. The most prevalent was the prognosis of average fertilization (53.3-58.0%), which was recorded in 62.9% of experimental sheep. The prognosis of high fertilization, which resulted in 62.5-65.1% of oseminins, was found in 27.8% of females. At the same time, the number of females with a fertility forecast at 40% was only 3.1%, and the prevalence of an unfavorable prognosis, in which fertility was the smallest (30.0-35.5%), reached 6.2%. Key words: sheep, askanian breeding, estrus, fertility prognosis, estral mucus, vulva, vagina, artificial insemination.
New criteria for cytological analysis of sheep vaginal swabs (cytovaginal examination) during sexual hunting, during the sexual cycle, after insemination and depending on the effectiveness of insemination have been developed. Cytological material was collected from the area of the upper vaginal vault using a sterile vaginal mirror, forceps and cotton gauze swab. Staining of brushstrokes was performed according to Gimza-Romanovsky. To improve the method of cytovaginal examination, the division of smear epitheliocytes into groups: functional, destroyed, non-nuclear and cells with nuclear pyknosis is proposed. In turn, among the functional cells it is advisable to distinguish between cells of the deep layers of the epithelium (basal-parabasal), intermediate and surface layer (superficial nuclear). The main types of ratio of particles of different groups and subgroups and their prognostic value in the mating season during sexual hunting are revealed. It was found that during the inhibition stage there is an intensive desquamation of the epithelial level, which was manifested by a 2.5-fold decrease in the number of basal / parabasal epitheliocytes and almost four - destroyed epitheliocytes. And already from the 13th day there is an increase in the cells of the functional level due to the proportion of cells of the deep and intermediate level. On the 18th day, the cellular composition of vaginal smears in sheep acquired characteristics characteristic of the stage of arousal. Thus, compared with the 6-8th day of the cycle, the number of destroyed epitheliocytes (p <0.001) and naked nuclei (up to 8.3 ± 1.6) doubled again (up to 12.0 ± 0.7) against the background of a decrease in 4 times (to the initial level) of cells with pyknosis of the nucleus. The main cytological sign of pregnancy from the fourth day after insemination was a 2.7-fold increase in the number of destroyed cells and "naked" nuclei. In the following days of the study, except for the 13th day, this difference ranged from 1.3 to 4.3 times (8.3 ± 5.4 to 14.7 ± 3.6%), compared with the corresponding figures in unfertilized ewes. Also on the 13th day after the introduction of sperm in fertilized females there is a tendency to increase the number of deep layer cells and the acquisition on the 18th day of a probable difference in the ratio between the number of deep and surface cells - 5: 1. Changes in the distribution of deep and surface cell particles in unfertilized animals indicate the manifestation of the next stage of excitation and the predominance of epithelial cell differentiation. In contrast, in pregnant females, the proliferation of the deep layers of the vaginal epithelium increased. Key words: ewes, sexual cycle, sexual hunting, vaginal smear, cytological composition, pregnancy.
It is established that the physiological course of the gestational process occurs with the intensification of coagulation processes in the hemostasis system with thrombophilia, which has a two–phase nature – from the first month of pregnancy to the seventh and in the period of 2–3 days to calving with a peak of hypercoagulation by 2–5 that day after birth. The activation of hemostasis components is correlated with a high level of natural anticoagulant protein C and increased fibrinolysis activity through tissue plasminogen activator, which is controlled by a high level of its type I inhibitor. The main hemostasiological criteria are the levels: in phase I – fibrinogen 4,9–6,1 g/l, soluble fibrin about 0,04 g/l, activated partial thromboplastin time 38,1–39,7 s, functionally inactive forms prothrombin 0,88 ± 0,2 mg/ml in the first month of pregnancy; in phase II – fibrinogen 5,1–7,0 g/l, soluble fibrin about 0,01 g/l, functionally inactive forms of prothrombin 0,17 ± 0,05 mg/ml and protein C 72,0 ± 1,4 % after childbirth. In obstetric and gynecological pathology, the hypercoagulable state is aggravated to consumption coagulopathy, which is reflected in high plasma levels of soluble fibrin – 0,064 ± 0,005 g/l, fibrin / fibrinogen cleavage products – 7,5 ± 0,4 μg/ml, functionally inactive forms of prothrombi. – 1,90 ± 0,34 mg/ml and inhibitor of tissue plasminogen activator type I – 45,8 ± 0,9 IU/ml at the lowest level of tissue plasminogen activator – 0,38 ± 0,08 IU/ml (according to the norms 1,1–1,3 IU/ml) and protein C deficiency – <70%. Hypercoagulation in pregnant cows with purulent– necrotic lesions of the extremities is exacerbated by more pronounced hyperfibrinogenemia, an increase in its metabolites by 2,7 times (p<0,05) and a decrease in the activity of F XIII by 1,3 times (p<0,05) and the amount in the blood of proteinase inhibitor α2–M – 1,6 times (p<0,05). At the same time, the hypercoagulation syndrome in cows with obstetric and gynecological pathology due to its comorbidity with orthopedic not only intensifies, which is expressed in an increase in the level of hyperfibrinogenemia by 1,2 times (p<0,05), reduction of general coagulation tests by 1,3–2,1 times (p<0,001) and a decrease in the activity of FXIII in 1,2–1,3 (p<0,05), but is complicated by a decrease in the antiproteinase potential of the blood in 1,6–1,8 times. Key words: cows, pregnancy, obstetric, gynecological pathology, purulent-necrotic lesions in the area of fingers, hemostasiological indicators.
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