Fiber Reinforced Polymer (FRP) bars are anisotropic in nature and have high tensile strength in the fiber direction. The use of High-Strength Concrete (HSC) allows for better use of the high-strength properties of FRP bars. The mechanical properties of FRP bars can yield to large crack widths and deflections. As a result, the design of concrete elements reinforced with FRP materials is often governed by the Serviceability Limit States (SLS). This study investigates the short-term serviceability behavior of FRP RC I-beams. Eight RC I-beams reinforced with carbon-FRP (CFRP) and four steel RC I-beams, for comparison purposes, were tested under two-point loading. Deformations on the concrete and crack widths and spacing are measured and analyzed. A discussion on the main aspects of the SLS of FRP RC is introduced. The service load that fulfills the serviceability requirements, at a cross-section level, ranges between 0.27 and 0.38 times the ultimate load for sections dimensioned to fail in concrete crushing. The determinant criterion is the deflection limitation