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Abstract. Long after its alleged demise, phlogiston was still presented, discussed and defended by leading chemists. Even some of the leading proponents of the new chemistry admitted its 'absolute existence'. We demonstrate that what was defended under the title 'phlogiston' was no longer a particular hypothesis about combustion and respiration. Rather, it was a set of ontological and epistemological assumptions and the empirical practices associated with them. Lavoisier's gravimetric reduction, in the eyes of the phlogistians, annihilated the autonomy of chemistry together with its peculiar concepts of chemical substance and quality, chemical process and chemical affinity. The defence of phlogiston was the defence of a distinctly chemical conception of matter and its appearances, a conception which reflected the chemist's acquaintance with details and particularities of substances, properties and processes and his skills of adducing causal relations from the interplay between their complexity and uniformity.

The optical instruments developed through the seventeenth century allowed peering into the very far and the very small; a spectacle never before experienced. The telescope, and later the microscope, was now expected to answer fundamental questions and resolve cosmological riddles by direct observation into the foundations of nature. But this ability came at an unexpected price and with unexpected results. For Kepler and Galileo, the new instruments did not offer extension and improvement to the senses; they replaced them altogether. To rely on their authority was to admit that the human eye is nothing but an instrument, and a flawed one at that. Rather than the intellect's window to the world, the human senses became a part of this world, a source of obscure and unreliable data, demanding uncertain deciphering. Accurate scientific observation meant that we are always wrong.