This study was carried out to evaluate the effects of chlorpyrifos and cypermethrin, singly and in combination on oxidative stress enzymes and biochemical indices of male albino rats. 35 male albino rats weighing 170-200 g were divided into seven groups (A-G) of five rats each in a Completely Randomized Design. Rats in group A served as the control and received feed and distilled water only. Groups B and C received 5 and 10 mg of chlorpyrifos; groups D and E received 5 and 10 mg of cypermethrin, while groups F and G received 5 and 10 mg of combination (chlorpyrifos+cypermethrin), respectively. Treatment was administered via oral gavage and lasted for 65 days. At the end of treatment, the rats were sacrificed and blood samples were analyzed for oxidative stress enzymes and biochemical indices. On the one hand, result obtained for oxidative stress enzymes revealed that pesticide treatment significantly (p<0.05) reduced the levels of superoxide dismutase (SOD), Glutathione Peroxidase (GPx) and Catalase (CAT) activity and increased lipid peroxidation (MDA) level in rats treated with chlorpyrifos only. On the other hand, result for biochemical indices revealed that aspartate transaminase (AST) increased significantly (p<0.05) in the group of rats treated with 10 mg of chlorpyrifos and 5 mg of cypermethrin and reduced in those treated with 5 mg of chlorpyrifos and 10 mg of cypermethrin. Alkaline phosphatase (ALP) level reduced significantly in all the groups except for the group that received 5 mg of chlorpyrifos. While, alkaline transaminase (ALT) level increased significantly in some treated rats especially in the group that received 10 mg of the combination. Present findings therefore demonstrate that chlorpyrifos and cypermethrin could induce oxidative stress and alter the levels of some liver marker enzymes in male rats.