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BackgroundThe aim of the study was to investigate the arabinoxylan (AX) content, yield, recovery, and enrichment factors of milling fractions obtained from short‐ and long‐flow milling from biofortified (+) and nonbiofortified (−) oats cv. Haskara by determining the monosaccharide components besides dietary fiber and mineral contents.FindingsCoarse brans (CB) obtained by short‐flow milling from Haskara (+) and (−) samples had around 3.3% AX contents and 1.5 enrichment factor while fine brans (FB) obtained by long‐flow milling had around 3.8% AX contents and enrichment factors higher than CB. The differences between bran samples of Haskara (+) and Haskara (−) were significant (p < .05) in terms of insoluble dietary fiber. CB of Haskara (+) had 1.5, 2.9, and 3.8 times higher Zn, I, and Se contents than those of whole grain Haskara (−) while FB of Haskara (+) had 1.7, 4.7, and 3.7 times higher Zn, I, and Se contents than those of whole grain Haskara (−), respectively.ConclusionsMineral/fiber‐rich fractions obtained by biofortification and milling applications can be used against mineral deficiency.Significance and NoveltyThis is a pioneering study on AX and mineral contents of fractions obtained by different milling flows from biofortified hull‐less oats.