Objectives: This study investigates the relationship between anxiety and headphone use. Materials and methods: The study was conducted on 180 (54 males, 126 females) undergraduate students. Headphone habits were assessed with a questionnaire, and anxiety levels were assessed with Beck Anxiety Inventory. Results: While there was no correlation between total anxiety scores and length of headphone use, there was a correlation with purpose of headphone use. There was a positive correlation between headphone use while using a computer (p<0.05), watching media, isolation from the environment, and while studying (p<0.01), as well as a positive correlation with headphone use in evening hours (p<0.05). According to the Beck scale, participants with scores between 26-63 had high anxiety levels and were positively correlated with headphone use in the evenings, headphone use at school, and when watching media (p<0.05). Conclusion: Our study, which aimed to investigate the relationship between headphone habits and anxiety, may inspire and guide further studies on this subject.