BACKGROUND: Hydrogel is a three-dimensional structure that has the potential to absorb and retain water within the mesh of its porous network structure. Currently hydrogels made from natural biopolymers are preferred in the discipline of biomedical applications because of their blood compatibility, adhesion of platelets and protein binding, ease of administration and delivery of ingredients to the place of action. OBJECTIVE: The aim of this work was to prepare a hydrogel from natural biopolymers and evaluate its blood compatibility, swelling nature, prolonged degradation and morphological features in order to further recommend its clinical use. METHODS: To prepare hydrogels, different combinations of gelatin, dialyzed SF, curcumin and N, N methylene bisacrylamide (MBA) were evenly mixed on a magnetic stirrer. After an hour of the gelation process it was kept in a refrigerator at 4 °C. For the characterization and biocompatibility studies of hydrogel, the swelling test and biodegradation analysis, SEM, FTIR, in vitro coagulation tests, total serum albumin and cholesterol level analysis were applied. RESULTS: Injectable hydrogels were successfully made with significantly correlated combinations of polymers. The analysis of physiochemical biocompatibility studies and morphological characterization were done effectively. CONCLUSION: The results of the study indicate that hydrogels made from natural biopolymers are a potential source and suitable matrices with excellent biocompatible nature acting as a useful device in delivering drugs.

Background Hydrogel is a structure with three-dimension that have the potential to absorb and retain water inside of it. Currently hydrogels made from natural biopolymers are preferred in the discipline of biomedicine due to their nature of blood compatibility, adhesion of platelets and protein binding, ease of administration and delivery of ingredients to the place of action. The purpose of this study is preparing blood compatible injectable hydrogels from naturally obtained polymers of biomaterials in a combination of different proportion, characterizing and analyzing its physiochemical blood compatibility and morphological structures. Methods Three different compositions of gelatin, dialyzed SF, curcumin and N, N methylene bisacrylamide (MBA) in different groups coded by IVA, IVB and IVC were used. The combinations were evenly mixed on a magnetic stirrer. After an hour of the gelation process it has been kept under refrigerator at 4°C. For characterization and biocompatibility studies of hydrogel swelling test and biodegradation analysis, SEM, FTIR, In-vitro coagulation tests, total serum albumin and cholesterol level analysis were applied, Results Injectable hydrogel successfully made from the combinations of SF/GE/CU/ in the presence of a cross linker MBA and the result from physiochemical biocompatibility and morphological characteristics study were confirmed that natural biopolymers used in this study are a candidate for biomedical applications. Conclusion The result confirmed that the composition coded by IVC was identified the most stable composition and suitable in its morphological structure with excellent blood compatible nature recommended for further biomedical applications.