Aim:The study is targeted at the sickle cell disease. It has been discovered that some drugs or medications taken for certain ailments are either pro-sickling or anti-sickling in nature. In this study, acorticosteroid by the name of prednisolone was investigated to determineits possible effects on human haemoglobin-S gelation, erythrocyte fragility and Fe 2+ and Fe 3+ concentrations. Materials and Method: The blood sample of 5ml was collected from adult male and female donors by vein puncture using a 5 ml syringe and needle. The blood samples were confirmed as HbSS using standard haemoglobin electrophoresis. Various concentrations of the drug (0.05, 0.1, 0.3, 0.5 and 1 mg/ml) were used to determine the effects on human haemoglobin-S, gelation rate, erythrocyte fragility, Fe 2+ & Fe 3+ concentrations. Absorbance reading was taken at 540 nm using a spectrophotometer. Results: The results showed that Prednisolone increased haemoglobin S gelation at all concentrations (p< 0.05) when compared to the control. The Fe 2+ /Fe 3+ ratio showed a reduction in Okoye et al.; JAMPS, 20(4): 1-7, 2019; Article no.JAMPS.47855 2 haemoglobin values at 0.3, 0.5 and 1.0 mg/ml concentrations when compared to the control and a slight increase at 0.05 and 0.1 mg/ml. For Erythrocyte Fragility, there was destabilization of red cell in all concentrations. Conclusion: This study suggests that this drug could have some undesirable effects on sickle cell subjects. Original Research Article