Introduction. The experimental examination of hypothesis about linear dependence of concentration polarization resistance from pressure was carried out and the influence of hydrodynamic condition on this resistance is determined. Materials and methods. The research was carried out with using of commercially available membrane modules TFC-75 type. The measurements of productivity were carried out with using of deionized water (total dissolved solids 5-15 mg/dm 3) and also NaCl solutions. The volumetric technique was used for flux measurements. The concentration was measured by conductometric technique. Results and discussion. The membrane resistance during reverse osmosis of deionized water did not change with applied pressure in experimental conditions and was equal Rm=7,549•10 13 m-1. The concentration polarization layer resistance (Rcp) increased from 0.65-1.29•10 13 m-1 to 1.46-1.83•10 13 m-1 with applied pressure increasing from 0.2 MPa to 0.6 MPa and from 0.65-1.46 m-1 to 1.29-1.83•10 13 m-1 with increasing of feed concentration from 100 mg/dm 3 to 600 mg/dm 3. This increasing of Rcp value with pressure was linear which is in agreement with previously reported data for the ultrafiltration process. Moreover, in considered range of applied pressure, the exponential dependence of index of concentration polarization from applied pressure could be approximated by a linear equation with correlation coefficient 0.93. Therefore, assumption about linear dependence of concentration polarization layer resistance from pressure is reasonable and could be extended to reverse osmosis process for mentioned above conditions. The increasing of concentration polarization layer resistance with increasing of applied pressure is determinated by higher values of transmembrane fluxes and lower values of mass transfer coefficient at higher values of applied pressure in the considered system. These results are in agreement with film theory of concentration polarization. Conclusions. The exanimated hypothesis is validated for reverse osmosis in considered range of applied pressure. The correlation between concentration polarization layer resistance and index of concentration polarization was defined.