Damping-off generally occurs in seedlings that have just germinated. This disease can cause severe damage, decay, and seedling death. The research was aimed to compute disease incidence and severity of damping-off and to identify the causal of damping-off disease in Paraserianthes falcataria. The survey was examined by using scoring with a 10% sampling intensity. The identification of morphological characteristics was examined macroscopically and microscopically. The result showed that the disease incidence most frequently occurred on 5-month-old seedlings in an open area of 75,00%, and the lowest was on 3-month-old seedlings in an open area of 13,40%. The immense severity of the damping-off attack occurred on 3-month-old seedlings in the greenhouse area of 37,78%, and the lowest occurred on 3-month-old seedlings in an open area of 2,84%. The causal of damping-off disease on P. falcataria in Permanent Nursery of IPB University was Rhizoctonia sp. Pathogenicity test toward P. falcataria seed showed 100% of disease infection. Rhizoctonia sp. caused seed decay hence inhibited seed germination.Keywords: damping-off, Rhizoctonia sp., sengon (Paraserianthes falcataria)
Endophytic fungi are the potential biological agent that could stimulate plant growth and inhibit plant disease. The existence of diverse and abundant endophytic fungi encourages continuous exploration. One of the plants that have potential as a source of endophytic fungi is balangeran (Shorea balangeran Korth.). The study aimed to identify the species of endophytic fungi from the leaf and twig of S. balangeran based on morphological characteristics. Fungi isolation was conducted by multiple sterilizations and purification. Furthermore, the macroscopic and microscopic morphological characteristics of the endophytic fungi isolates were also identified. The results showed nine colony characteristics of endophytic fungi. Endophytic fungi of Colletotrichum sp1. (SbD 1.1), Phomopsis sp. (SbD 1.3.1), Colletotrichum sp2. (SbD 1.3.2), and Beauveria sp. (SbD 3.1) were only found on the leaves, while Aspergillus niger (SbB 5.1), Colletotrichum sp3. (SbB 5.2), and Nigrospora sp. (SbB 5.3.2 and SbB 6.3) were only found on the twigs. Endophytic fungi Phyllosticta sp. (SbD 1.2) were found in the leaves and twigs. The growth rate of endophytic fungi showed that Colletotrichum sp3 (SbB 5.2) was the fastest, and Phyllosticta sp. (SbD 1.2) was the slowest among the nine isolates. Endophytic fungi that have been isolated will be analyzed for their benefits as a biological agent in future research. Keywords: endophytic fungi, identification, morphological characterization, Shorea balangeran
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