Microsomal esterases were used in the study of the in vitro enzymic hydrolysis of the ester bond in alkyl(2-benzoyloxyethyl)dimethylammonium bromides. These compounds are potential "soft" disinfectants, easily biodegradable to nontoxic biologically inactive hydrolytic products, namely substituted choline and benzoic acid. Formation of the latter product was used to monitor the kinetics of the reaction. It has been found that the rate of enzymic hydrolysis is substantially influenced by different length of the alkyl chain on the ammonium nitrogen. At the same time, interspecies (rat-mouse) and interorgan (liver-kidney) variability has been observed.

biochemical syntheses, microbiological syntheses biochemical syntheses, microbiological syntheses O 0035 -057Dependence of in vitro Enzymic Hydrolysis of Alkyl(2benzoyloxyethyl)dimethylammonium Bromides on the Alkyl Length.-Microsomal esterases are used in the study of the in vitro enzymic hydrolysis of the ester bond in the title compounds, which prove to be potential "soft" disinfectants. They exhibit high activity against the microorganisms tested. An activity increase with the alkyl chain length is found with best results in the range from decyl to octadecyl chains. -(OLASZOVA, E.; PAULIKOVA, I.; HELIA, O.; SVAJDLENKA, E.; DEVINSKY, F.; LACKO, I.; Collect. Czech.