Fluorescent staining is a common tool for both quantitative and qualitative assessment of pro- and eukaryotic cells sub-population fractions by using microscopy and flow cytometry. However, direct cell counting by flow cytometry is often limited, for example when working with cells rigidly adhered either to each other or to external surfaces like bacterial biofilms or adherent cell lines and tissue samples. An alternative approach is provided by using fluorescent microscopy and confocal laser scanning microscopy (CLSM), which enables the evaluation of fractions of cells subpopulations in a given sample. For the quantitative assessment of cell fractions in microphotographs, we suggest a simple two-step algorithm that combines single cells selection and the statistical analysis. To facilitate the first step, we suggest a simple procedure that supports finding the balance between the detection threshold and the typical size of single cells based on objective cell size distribution analysis. Based on a series of experimental measurements performed on bacterial and eukaryotic cells under various conditions, we show explicitly that the suggested approach effectively accounts for the fractions of different cell sub-populations (like the live/dead staining in our samples) in all studied cases that are in good agreement with manual cell counting on microphotographs and flow cytometry data. This algorithm is implemented as a simple software tool that includes an intuitive and user-friendly graphical interface for the initial adjustment of algorithm parameters to the microphotographs analysis as well as for the sequential analysis of homogeneous series of similar microscopic images without further user intervention. The software tool entitled BioFilmAnalyzer is freely available online at https://bitbucket.org/rogex/biofilmanalyzer/downloads/.

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Understanding the physical principles that govern the complex DNA structural organization as well as its mechanical and thermodynamical properties is essential for the advancement in both life sciences and genetic engineering. Recently we have discovered that the complex DNA organization is explicitly reflected in the arrangement of nucleotides depicted by the universal power law tailed internucleotide interval distribution that is valid for complete genomes of various prokaryotic and eukaryotic organisms. Here we suggest a superstatistical model that represents a long DNA molecule by a series of consecutive ~150 bp DNA segments with the alternation of the local nucleotide composition between segments exhibiting long-range correlations. We show that the superstatistical model and the corresponding DNA generation algorithm explicitly reproduce the laws governing the empirical nucleotide arrangement properties of the DNA sequences for various global GC contents and optimal living temperatures. Finally, we discuss the relevance of our model in terms of the DNA mechanical properties. As an outlook, we focus on finding the DNA sequences that encode a given protein while simultaneously reproducing the nucleotide arrangement laws observed from empirical genomes, that may be of interest in the optimization of genetic engineering of long DNA molecules.

We consider different antenna configurations, ranging from simple X-configuration to multi-beam star geometries, for airborne scatterometric measurements of the wind vector near the ocean surface. For all geometries, track-stabilized antenna configurations, as well as horizontal transmitter and receiver polarizations, are considered. The wind vector retrieval algorithm is generalized here for an arbitrary star geometry antenna configuration and tested using the Ku-Band geophysical model function. Using Monte Carlo simulations for the fixed total measurement time, we show explicitly that the relative wind speed estimation accuracy barely depends on the chosen antenna geometry, while the maximum wind direction retrieval error reduces moderately with increasing angular resolution, although at the cost of increased retrieval algorithm computational complexity, thus, limiting online analysis options with onboard equipment. Remarkably, the simplest X-configuration, while the simplest in terms of hardware implementation and computational time, appears an outlier, yielding considerably higher maximum retrieval errors when compared to all other configurations. We believe that our results are useful for the optimization of both hardware and software design for modern airborne scatterometric measurement systems based on tunable antenna arrays especially, those requiring online data processing.

We consider sea ice and water microwave backscatter features at the C-band with vertical transmit and receive polarization and present a method for sea ice/water discrimination using a multiple fixed fan-beam satellite scatterometer. The method is based on the criterion of the minimum statistical distance of measured backscatter values to the sea ice and water (CMOD7) geophysical model functions. Implementation of the method is considered both for a typical three fan-beam geometry as well as for a potential five fan-beam geometry of a satellite scatterometer. By using computer simulations, we show explicitly that the number of looks at the same cell from different azimuthal directions needs to be increased to provide better (unambiguous) retrieval of the wind vector and sea ice/water discrimination. The algorithms for sea ice/water discrimination are described, and the results obtained are also discussed along with recommendations for the number of different azimuthal looks (beams) at the same cell from the point of view of sea ice/water discrimination as well as unambiguous wind direction retrieval during the satellite’s single pass.