Patients can present with infections of the skin and soft tissues due to the pathogen Mycobacterium chelonae. Clinical presentation of this relatively rare infectious disease may differ from individual to individual. Despite an increasing incidence, affected patients are often misdiagnosed. Thus, in addition to careful history-taking, tissue biopsies for mycobacterial culture and histopathological examination are needed to reach a correct diagnosis. Once diagnosed, culture-directed antibiotic therapy is required. We report here 5 patients with cutaneous and/or soft-tissue infections due to M. chelonae, who were diagnosed and treated appropriately at our centre. Mycobacterium chelonae is a rapidly growing nontuber culous mycobacterium, which causes infections of the human skin and soft tissue. Despite an increasing incidence of such infections, patients are often misdiagnosed. We report here 5 patients with cutaneous and/ or soft tissue infection due to M. chelonae who were diagnosed and treated at our centre. Two of the 5 patients were on immunosuppressive treatment. While clinical presentations differed in each patient, all had a long history of skin lesions. In addition to careful history-taking, tissue biopsies were obtained for mycobacterial culture and histopathological examination. Culturedirected antibiotic therapy was initiated, which resulted in a slow, but continuous, healing of the lesions. In summary, M. chelonae infections are still relatively rare, but should be considered in both immunocompromised and immunocompetent patients with prolonged skin lesions resistant to standard antibiotic treatment. For diagnosis, tissue analysis for mycobacterial culture and histopathological examination, and once diagnosed, adequate antibiotic treatment, is needed.