Recently, photoactive proteins have
gained a lot of attention due
to their incorporation into bioinspired (photo)electrochemical and
solar cells. This paper describes the measurement of the asymmetry
of current transport of self-assembled monolayers (SAMs) of the entire
photosystem I (PSI) protein complex (not the isolated reaction center,
RCI), on two different “director SAMs” supported by
ultraflat Au substrates. The director SAMs induce the preferential
orientation of PSI, which manifest as asymmetry in tunneling charge-transport.
We measured the oriented SAMs of PSI using eutectic Ga–In (EGaIn),
a large-area technique, and conducting probe atomic force microscopy
(CP-AFM), a single-complex technique, and determined that the transport
properties are comparable. By varying the temperatures at which the
measurements were performed, we found that there is no measurable
dependence of the current on temperature from ±0.1 to ±1.0
V bias, and thus, we suggest tunneling as the mechanism for transport;
there are no thermally activated (e.g., hopping)
processes. Therefore, it is likely that relaxation in the electron
transport chain is not responsible for the asymmetry in the conductance
of SAMs of PSI complexes in these junctions, which we ascribe instead
to the presence of a large, net dipole moment present in PSI.
HIGHLIGHTSJunction geometry determines effective contact area Mechanism of charge transport is independent of junction platform Electrode-molecule coupling determines transport efficiency across interfaces Tunneling dominates solid-state electron transport across proteinbased junctions
Interfacing proteins with electrode surfaces is important for the field of bioelectronics. Here, a general concept based on phage display is presented to evolve small peptide binders for immobilizing and orienting large protein complexes on semiconducting substrates. Employing this method, photosystem I is incorporated into solid‐state biophotovoltaic cells.
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a genomics-driven workflow to assemble the complete MPAO1 genome, identify unique and conserved genes by comparative genomics with the PAO1 reference strain and genes missed within existing assemblies by proteogenomics. Among over 200 unique MPAO1 genes, we identified six general essential genes that were overlooked when mapping public Tn-seq data sets against PAO1, including an antitoxin. Genomic data were integrated with phenotypic data from an experimental workflow using a user-friendly, soft lithography-based microfluidic flow chamber for biofilm growth and a screen with the Tn-mutant library in microtiter plates. The screen identified hitherto unknown genes involved in biofilm growth and antibiotic resistance. Experiments conducted with the flow chamber across three laboratories delivered reproducible data on P. aeruginosa biofilms and validated the function of both known genes and genes identified in the Tn-mutant screens. Differential protein abundance data from planktonic cells versus biofilm confirmed the upregulation of candidates known to affect biofilm formation, of structural and secreted proteins of type VI secretion systems, and provided proteogenomic evidence for some missed MPAO1 genes. This integrated, broadly applicable model promises to improve the mechanistic understanding of biofilm formation, antimicrobial tolerance, and resistance evolution in biofilms.
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