Inter-limb asymmetry is defined as the performance imbalance of a limb with respect to the other one. Studies demonstrated how plyometric training could minimize inter-limb imbalance by reducing asymmetries and the relative risk of musculoskeletal injuries. Among the different plyometric training modalities, SuperJump® represented a reliable method. This study aimed to evaluate the acute effects of SuperJump® training on dynamic balance Unilateral Asymmetry and Bilateral Asymmetry Indexes. Thirty-seven subjects were randomly allocated in two groups: SuperJump® (N = 20) and Control (N = 17). The SuperJump® group participated in the SuperJump® session, whereas the Control group did not receive any workout session. Before (PRE) and after (POST) the SuperJump® session, both groups performed the Wobble Board balance test. A significant difference (p < 0.003) between the SuperJump® in POST and Control groups in PRE intervention for dominant leg was found. No significant differences (p > 0.05) emerged between groups in testing time on Bilateral Asymmetry Index. Significant differences (p = 0.005) between PRE and POST in the SuperJump® group and an 18.9% Unilateral Asymmetry Index threshold reduction for the subjects were found. A strong relationship (R2 = 0.79) between delta (∆) change Unilateral Asymmetry Index and Unilateral Asymmetry Index baseline parameters was tested. Lastly, no significant differences (p > 0.05) in Unilateral Asymmetry Indexes between the Bilateral Asymmetry equal and change categories in the SuperJump® group was found. In conclusion, SuperJump® training played a key role in reducing Unilateral Asymmetry Index.