The aim of this study was to estimate the sensitivity of microorganisms, isolated from soil long-term polluted with obsolete pesticides, to magnetite (Fe3O4) nanoparticles and fluorinated dinitroaniline herbicide trifluralin, and to evaluate the inhibition activity of these substances. The response of fungi and streptomycete strains to the presence of magnetite nanoparticles in culture media is individual to each microorganism. For the most of studied microorganisms, the addition of trifluralin to culture media had a growth inhibition effect. An exception was the strain Streptomyces sp. 0412, which growth was stimulated in the presence of xenobiotic. The negative effect of pesticide was reduced, when before the addition to the culture medium the trifluralin was mixed and incubated with magnetite nanoparticles for 1 hour.

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Six agricultural organic wastes and three inorganic matrices were selected for rhodococci whole cells immobilization. The degree of immobilization of rhodococci cells varied from 6.20% to 34.30% on organic matrices. A high level of Rhodococcus rhodochrous CNMN-Ac-05 cells immobilization was demonstrated on inorganic matrices, it was from 69.25% to 97.30%. After the contact with support the strain dissociated, forming, in addition to original S type, rough (R) and altercolour smooth (S) types. Immobilization of rhodococci cells on organic supports led to the appearance of phenotypic heterogeneity from 0.34% to 3.26%. On inorganic matrices the variability of rhodococci was 0.88-1.05%.