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Introduction. Bacterial vaginosis (BV) is the primary cause of pathological vaginal discharge in women of reproductive age. Gardnerella vaginalis and Atopobium vaginae are considered key components of the vaginal microflora in BV. Etiology, pathogenesis and modes of transmission of BV are actively studied, however these questions still remain unanswered. Objective: investigate predictor factors of BV in women with vaginal discharge. Material and methods. In total, 318 women were included. As clinical material, vaginal samples were used. BV was diagnosed using the Nugent method. For quantitative determination of G. vaginalis and A. vaginae DNA, real-time PCR was used. Behavioral and anamnestic data were obtained from questionnaire filled out by the patients. Results. BV was diagnosed in 27 % of women. G. vaginalis and A. vaginae DNA was detected, respectively, in 93 % and 83 % of patients with BV, 73 % and 59 % - with intermediate microflora, 52 % and 38 % - with normal microflora. Difference between the three types of microflora in the frequency and concentrations of these microorganisms were statistically significant. Detection of G. vaginalis and A. vaginae were significant predictor factors of BV (OR 12.2; 95 % CI 5.1-29.4 and OR 7.9; 95 % CI 4.2-14.9, respectively), with chances to diagnose BV being manifold increased when clinically significant concentrations of these bacteria were detected (≥3×106 and ≥8×105 DNA copies/ml for G. vaginalis and A. vaginae, respectively). Detection of clue cells in Gram stained preparations was shown to be the strongest BV predictor (OR 765.6; 95 % CI 99.6-5883.2). Conclusions. BV is diagnosed in more than one fourth of women with vaginal discharge. Detection of G. vaginalis and A. vaginae, especially in clinically significant concentrations, and clue cells in Gram stained preparations are significant predictor factors of BV.

The prevalence of syphilis with RP and TPHA was 0.9% (12/1400). RTs for syphilis showed > 90% sensitivity and 100% specificity. RTs for C trachomatis showed a low sensitivity between 22 -37% and a 99% specificity, RTs for N gonorrhoeae showed 97%. Conclusions In women with symptoms of LGTIs RTs used at the point of care for syphilis have a sensitivity > 90%. RTs for CT have sensitivity < 40% and Rts for NG have sensitivity < 12.5% %.Funded Background Young people are worldwide a risk group for sexually transmitted infections (STIs) and a primary target for screening. Knowledge on STI prevalence in the youths is essential to elaborate preventive measures. Self-sampling has been shown to be an effective approach in screening and epidemiological programmes. This study aimed to assess the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Trichomonas vaginalis in young people in St. Petersburg, Russia using self-collected non-invasive specimens. Methods In total, 1207 consecutive sexually active attendees (1053 female and 154 male) of the youth centre Yuventa in St. Petersburg, Russia, aged 15-25 years and consenting to participate, were enrolled in the study from June through November 2011. The mean age of the women was 20.2 ± 2.8 years, and the men 20.2 ± 2.9 years. Vaginal and male urine samples were self-collected using SelfCollection Specimen Kit (Central Research Institute for Epidemiology, Russia) and UriSWAB (Copan, Italy), respectively. Testing for the STIs was performed by multiplex real-time PCR (AmpliSens N.gonorrhoeae/C.trachomatis/M.genitalium/T.vaginalis-MUL-TIPRIME-FRT, Central Research Institute for Epidemiology). Results The overall prevalence of the examined STIs was 8.1% (85 of 1053) in the women and 7.8% (12 of 154) in the men. C. trachomatis, N. gonorrhoeae, M. genitalium and T. vaginalis were detected in 70 (6.6%), 6 (0.6%), 12 (1.1%) and 3 (0.3%) women, respectively. The prevalence of C. trachomatis and M. genitalium in the men was 6.5% (10 of 154) and 1.3% (2 of 154). N. gonorrhoeae or T. vaginalis were not detected in any men. In 7 women, multiple agents were found, i.e., C. trachomatis and N. gonorrhoeae (n = 3), C. trachomatis and M. genitalium (n = 2), and M. genitalium and T. vaginalis (n = 1). laboraTory diagnoSiS of geniTal P2.022Background The knowledge about approaches used for diagnosis of STIs in Ukraine is scarce. Aiming to optimise the laboratory diagnosis of STIs and introduce antimicrobial resistance surveillance for Neisseria gonorrhoeae, we aimed to survey the algorithms, methodologies and reagents used, and the laboratory capacities and possibilities in three regions of Ukraine. Methods Laboratories of three regions of Ukraine, namely Dnepropetrovsk, Ternopil and Zaporoz, were visited and detailed interviews were conducted. Results The three main dispensaries visited serve both the corresponding region as well as the city needs, and also have their own outpatient clinics. Large number of samples is tested, for example in Dnepropetrovsk an...

Introduction. Bacterial vaginosis (BV) is associated with a number of reproductive health disorders, therefore timely and accurate diagnosis of this condition is exceedingly important. Objective.Comparison of effectiveness of clinical and laboratory diagnostics of BV in women with vaginal discharge. Material and methods. In total, 318 patients addressing gynecological clinics with complaints about vaginal discharge participated in the study. Clinical diagnostics of BV was performed in the clinics participating in patient enrollment in accordance with their clinical practice. For laboratory diagnostics, microscopy of Gram stained smears according to the Nugent method and quantitative real-time PCR were used. Sensitivity and specificity of clinical diagnostics of BV and the molecular method were evaluated using the Nugent method as reference standard. Results. With the Nugent method, BV was diagnosed in 27% of women, with real-time PCR — in 37% of women. Using clinical signs of BV, the condition was diagnosed in 91% women. Sensitivity and specificity of the real-time PCR were 97% and 87%, respectively. Sensitivity of clinical diagnostics was 100%, but specificity was only 17%. Conclusions. Diagnostics of BV based only on the presence of vaginal discharge leads to false positive results and requires laboratory confirmation. The molecular method has a high sensitivity and satisfactory specificity for BV diagnosis and can be used as an alternative to the Nugent method.

The paper presents data on comparison of microscopy, culture and molecular (PCR) methods for diagnosis of trichomoniasis, as well as on evaluation of the quality of culture media for isolation of trichomonads produced by Russian and international manufacturers. The highest sensitivity was shown for the molecular (real-time PCR) method. Microscopy of stained and wet smears demonstrated relatively high sensitivity. The culture media that are widely used in our country for Trichomonas vaginalis isolation need optimization.