Introduction: Over the past decade, preeclampsia has been the subject of an increased attention, as this complication is the most common cause of maternal mortality, triggering every third case of obstetric morbidity and taking lives of up to 50.000 young women worldwide each year. Despite a large number of ongoing studies, no clear algorithm for monitoring pregnant women with this pathology has been developed yet. Materials and methods: The study was conducted on 220 female Wistar rats weighing 250–300 g. In the experiment, ADMA-like preeclampsia model was used. Results and discussion: The introduction of L-NAME to the animals from the 14th till the 20th day of pregnancy causes the following disturbances: a rise in systolic and diastolic blood pressure in 1.4 and 1.5 times, an increase in proteinuria in 3.3 times and an increase in the content of fluid in the greater omentum from 44.39 ± 1.62% to 55.02 ± 1.74%, all of which correspond to the disorders in pregnant women in case of preeclampsia. The use of the selective inhibitor of arginase II KUD 975 (3mg/kg/day) in combination with acetylsalicylic acid (10mg/kg/day) leads to a pronounced correction in the emerging changes: a decrease in systolic and diastolic blood pressure in 1.2 and 1.3 times, a decrease in proteinuria in 1.9 times and a decrease in the fluid content in the greater omentum. Conclusion: Selective inhibitor of arginase II KUD 975 and small doses of acetylsalicylic acid have a pronounced positive effect in the correction of morphofunctional disorders in animals with ADMA-like preeclampsia.

Background: Hypertensive disorders, in particular preeclampsia, which accounts for 2 to 8% of the total number of pregnancies in the world, are one of the leading causes of serious complications, prolonged disability and death in mothers and babies. In the structure of perinatal mortality, hypertensive disorders during pregnancy account for up to 25% of the total number of cases, and also occupy the leading places in the structure of causes of maternal mortality. The aim of the study: to study the protective activity of a small dose of acetylsalicylic acid and its combination with methyldopa in the correction of ADMA-like preeclampsia. Materials and methods: The study was performed on 150 female Wistar rats weighing 250-300 g. An ADMA-like model of preeclampsia was reproduced in the experiment. The following parameters were used to assess emerging morphofunctional disorders like blood pressure, coefficient of endothelial dysfunction, microcirculation in the placenta, proteinuria, fluid content in the greater omentum, concentration of final metabolites in blood plasma, fetal morphometric parameters. Results: When assessing morphofunctional disorders in animals with ADMA-like preeclampsia, the study has revealed a positive effect of a small dose of acetylsalicylic acid in combination with methyldopa. Conclusion:The combined administration of a small dose of acetylsalicylic acid and methyldopa has a pronounced protective activity in the correction of morphofunctional disorders in animals with ADMA-like preeclampsia and is a promising direction in the treatment of hypertensive disorders during pregnancy.

Introduction: The disruption of the functional state of the vascular endothelium is among the main causes of preeclampsia, which is one of the most common causes of maternal and perinatal mortality. It can be enhanced by the humoral factors secreted by the activated platelets. The use of acetylsalicylic acid is an effective way to prevent preeclampsia. However, its ability to activate eNOS is a prerequisite for researching its ability to correct the disorders in developing preeclampsia, including by reducing the platelet activity. In this case its effect can be enhanced through increasing the bioavailability of L-arginine by using a selective arginase II inhibitor KUD 975. These facts were the prerequisite for conducting this study. Materials and methods: The study was conducted on 180 female Wistar rats weighing 250–300 g. Acetylsalicylic acid was used at a dose of 7 mg/kg/day and 10 mg/kg/day, KUD 975 – at a dose of 1 mg/kg/day and 3 mg/kg/day. Adenosine diphosphate (ADP, 6.5 microns), arachidonic acid (ASPI, 0.5 mM), and collagen (3.2 mcg/ml) were used as aggregation inducers. Results and discussion: ADMA-like preeclampsia simulation led to an increase in platelet aggregation ability when using all aggregation inducers. This is evidenced by an increase in a degree, rate of aggregation, and a shortened time of thrombus formation. The use of acetylsalicylic acid and a selective arginase II inhibitor KUD 975 led to a decrease in the aggregation ability of platelets and an increase in thrombosis time, while the combined administration of the studied agents showed a more pronounced effect. Conclusion: The data obtained while performing a series of experiments strongly indicate a promising outlook for using acetylsalicylic acid and a selective arginase II inhibitor KUD 975 in order to correct emerging disorders in preeclampsia.