Background. The aim of the study was to find out the influence of excess copper and zinc ions in drinking water on the bile-forming and bile-secreting functions of the liver in the conditions of mechanical trauma and the effectiveness of the antidote therapy. Material and methods. The experiments were performed on 60 Wistar strain male rats weighing 180-200 g. There were 3 groups of experimental animals in the experiment: 1 group -only skeletal trauma was simulated; 2 group -a skeletal injury was simulated and zinc and copper salts were administered; 3 group -skeletal trauma + zinc and copper salts followed by Unithiol treatment. Results. In the late period of traumatic illness (14-28 days), after a severe skeletal injury, the intensity of bromsulfalein increased in the liver tissue, and there were signs of increased formation of direct bilirubin in terms of additional intake of copper and zinc ions. The animals with severe skeletal trauma were accompanied by a significant deterioration in the functional state of the liver in comparison to the animals with similar skeletal trauma without additional intoxication. Conclusions. Under conditions of additional intake of copper and zinc ions (in the 2nd group), after 14 days and 28 days of observation, all the studied indicators are much higher than in the group of the animals without additional intoxication with these ions. The use of Unithiol reduced the toxic effect of copper and zinc ions on the studied indicators of the functional state of the liver both in the early and late period of the traumatic disease.

Background. The aim of this work was to determine the effect of the combined action of nanoparticles of stearates and lead on the state of lipid peroxidation, antioxidant defense, and the level of circulating immune complexes in experimental rats. Material and methods. Experiments were conducted on four groups of white rats. The first (control) and second groups of animals consumed dechlorinated water from the city water supply. The third and fourth groups of animals consumed water, respectively, with the content of sodium stearate and potassium stearate in a dose of 1/250 LD 50 . After 40 days of drinking the indicated waters, the animals of the second, third, and fourth groups were orally injected with lead nanoparticles at a dose of 1/250 of the LD 50 .Results. The combined effect of sodium stearate and lead nanoparticles, potassium stearate, and lead nanoparticles had the character of additive action and potentiation according to most indicators of lipid peroxidation and antioxidant protection. According to the change of the circulating immune complexes indicator in the blood, it had the character of antagonistic action.Conclusions. The studies have shown that, in most indicators of lipid peroxidation and antioxidant system, the combined action of lead nanoparticles and stearates had the character of potentiation.