Introduction: Diabetes Mellitus (DM) is a multifactorial disease with high worldwide progression. In view of this scenario, an evaluation of future professionals who will compose the front line in assistance provided to people is justified. Objective: to identify the knowledge of students from different health courses regarding DM and the specific care actions of each professional area. Methodology: quantitative descriptive exploratory study, carried out in an HEI in the Federal District. Students from health courses were evaluated. Result/Discussion: There was a higher prevalence of females; aged between 18 and 24 years. In the evaluation categories that addressed issues of Specific Knowledge, Treatment and Complications of DM, the Physical Education and Nursing courses stood out. Conclusion: It can be concluded that 5 of the 7 courses in the health area of this HEI achieved scores referring to expected/desired scientific knowledge that demonstrate training for the clinical management of diabetic patients.