Alpha-arbutin (4-hydroxyphenyl alpha-glucopyranoside) is a known inhibitor of tyrosinase in keratinocytes; however, its effect on other genes and pathways in other skin cells has not been thoroughly investigated. In this study, we investigate the mechanism of alpha-arbutin activity in human dermal fibroblast cultures for 48 h. Results showed that the oxidative stress pathway was activated as alpha-arbutin reduced reactive oxygen species. In addition, we found a high possibility of wound healing and the upregulation of the insulin-like growth factor 1 receptor (IFG1R) pathway. We also investigated the role of the NRF2 gene in mediating the alpha-arbutin response. In silico comparative genomics analysis conducted using our original tool, SHOE, suggested transcription factors with a role in tumor suppression and toxicity response as candidates for regulating the alpha-arbutin–mediated pathway.

Background Assistive robotic hand orthoses can support people with sensorimotor hand impairment in many activities of daily living and therefore help to regain independence. However, in order for the users to fully benefit from the functionalities of such devices, a safe and reliable way to detect their movement intention for device control is crucial. Gesture recognition based on force myography measuring volumetric changes in the muscles during contraction has been previously shown to be a viable and easy to implement strategy to control hand prostheses. Whether this approach could be efficiently applied to intuitively control an assistive robotic hand orthosis remains to be investigated. Methods In this work, we assessed the feasibility of using force myography measured from the forearm to control a robotic hand orthosis worn on the hand ipsilateral to the measurement site. In ten neurologically-intact participants wearing a robotic hand orthosis, we collected data for four gestures trained in nine arm configurations, i.e., seven static positions and two dynamic movements, corresponding to typical activities of daily living conditions. In an offline analysis, we determined classification accuracies for two binary classifiers (one for opening and one for closing) and further assessed the impact of individual training arm configurations on the overall performance. Results We achieved an overall classification accuracy of 92.9% (averaged over two binary classifiers, individual accuracies 95.5% and 90.3%, respectively) but found a large variation in performance between participants, ranging from 75.4 up to 100%. Averaged inference times per sample were measured below 0.15 ms. Further, we found that the number of training arm configurations could be reduced from nine to six without notably decreasing classification performance. Conclusion The results of this work support the general feasibility of using force myography as an intuitive intention detection strategy for a robotic hand orthosis. Further, the findings also generated valuable insights into challenges and potential ways to overcome them in view of applying such technologies for assisting people with sensorimotor hand impairment during activities of daily living.

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Ankle joint power is usually determined by a complex process that involves heavy equipment and complex biomechanical models. Instead of using heavy equipment, we proposed effective machine learning (ML) and deep learning (DL) models to estimate the ankle joint power using force myography (FMG) sensors. In this study, FMG signals were collected from nine young, healthy participants. The task was to walk on a special treadmill for five different velocities with a respective duration of 1 min. FMG signals were collected from an FMG strap that consists of 8 force resisting sensor (FSR) sensors. The strap was positioned around the lower leg. The ground truth value for ankle joint power was determined with the help of a complex biomechanical model. At first, the predictors' value was preprocessed using a rolling mean filter. Following, three sets of features were formed where the first set includes raw FMG signals, and the other two sets contained time-domain and frequency-domain features extracted using the first set. Cat Boost Regressor (CBR), Long-Short Term Memory (LSTM), and Convolutional Neural Network (CNN) were trained and tested using these three features sets. The results presented in this study showed a correlation coefficient of R = 0.91 ± 0.07 for intrasubject testing and were found acceptable when compared to other similar studies. The CNN on raw features and the LSTM on time-domain features outperformed the other variations. Aside from that, a performance gap between the slowest and fastest walking distance was observed. The results from this study showed that it was possible to achieve an acceptable correlation coefficient in the prediction of ankle joint power using FMG sensors with an appropriate combination of feature set and ML model.

Background: Assistive robotic hand orthoses can support people with sensorimotor hand impairment in many activities of daily living and therefore help to regain independence. However, in order for the users to fully benefit from the functionalities of such devices, a safe and reliable way to detect their movement intention for device control is crucial. Gesture recognition based on force myography measuring volumetric changes in the muscles during contraction has been previously shown to be a viable and easy to implement strategy to control hand prostheses. Whether this approach could be efficiently applied to intuitively control an assistive robotic hand orthosis remains to be investigated. Methods: In this work, we assessed the feasibility of using force myography measured from the forearm to control a robotic hand orthosis worn on the hand ipsilateral to the measurement site. In ten neurologically-intact participants wearing a robotic hand orthosis, we collected data for four gestures trained in nine arm configurations, i.e., seven static positions and two dynamic movements, corresponding to typical activities of daily living conditions. In an offline analysis, we determined achieved classification accuracies for two binary classifiers (one for opening and one for closing) and further assessed the impact of individual training arm configurations on the overall performance. Results: We achieved an overall classification accuracy of 92.9% (averaged over two binary classifiers, individual accuracies 95.5% and 90.3%, respectively) but found a large variation in performance between participants, ranging from 75.4% up to 100%. Further, we found that the number of training arm configurations could be reduced from nine to six without notably decreasing classification performance. Conclusion: The results of this work support the general feasibility of using force myography as an intuitive intention detection strategy for a hand orthosis. Further, the findings also generated valuable insights into challenges and potential ways to overcome them in view of applying such technologies for assisting people with sensorimotor hand impairment during activities of daily living.