The retina is a tissue placed on the posterior surface of the eye that encodes the visual information from the environment. This code is represented by action potentials or spikes transmitted along the optic nerve to the brain, whose characteristics depend on the visual stimulus that come to the retina. Currently, it is not still completely clear which coding strategies are employed by the retina to encode the visual information, although some of them have been assumed in the recent years. The present work evaluates some coding strategies to reconstruct visual stimuli corresponding to sequences of four light intensities randomly presented. This task was performed with combinations of three coding strategies: latency, first inter-spike interval and firing rate. The classification was based on linear discriminate analysis, with the aim to determine which combinations of coding strategies detect the information of the light intensities. The performance of the method depended on the coding strategies combinations and the specific light intensity to reconstruct.

<p>Las matrices de microelectrodos son dispositivos que permiten la detección de potenciales de acción o espigas en poblaciones de células excitables, ofreciendo varias aplicaciones en el campo de las neurociencias y la biología. Este trabajo muestra un protocolo para el registro de espigas en una población de células ganglionares retinales empleando una matriz de microelectrodos. La retina de una rata albina fue extraída y preparada para ser estimulada <em>in vitro </em>con luz led blanca, con el fin de registrar sus espigas evocadas ante estos estímulos. Cada microelectrodo puede registrar espigas de más de una célula ganglionar, razón por la cual se determinó a qué célula pertenece cada espiga aplicando un procedimiento conocido como “clasificación de espigas”. El trabajo permitió obtener el registro de un periodo de estimulación y otro de no estimulación, con el fin de representar los potenciales de acción evocados con luz y los espontáneos. Los registros fueron almacenados para visualizar las espigas de las células ganglionares y poder aplicar la herramienta de clasificación de espigas. De este modo, se almacenan los instantes de tiempo en los cuales cada célula ganglionar registrada generó potenciales de acción. Este trabajo conllevó al establecimiento de un protocolo de experimentación básico enfocado al uso de matrices MEA en el laboratorio de adquisición de potenciales extracelulares de la Universidad Antonio Nariño Sede Bogotá, no sólo para caracterizar los potenciales de acción de células ganglionares retinales, sino también para otro tipo de células que puedan ser estudiadas empleando matrices de microelectrodos.</p><p align="center"><strong>Recording of Electrical Activity in the Retina of an Albino Rat Employing a Microelectrode Array</strong></p><p>The microelectrode arrays (MEA) are devices that allow the detection of action potentials or spikes in populations of excitable cells, offering a wide spectrum of applications in topics of Neurosciences and Biology. This work describes a protocol for recording of spikes in a population of retinal ganglion cells employing a microelectrode array. The retina of an albino rat was dissected and prepared to be stimulated<em> in vitro </em>with white led light and to record their evoked spikes. Each microelectrode can record spikes from more than a ganglion cell, for which it was necessary to determine which cell fires each spike applying a procedure known as spike sorting. The work allowed to obtain the recording of a stimulation period and another of non-stimulation, representing evoked and spontaneous action potentials. The recordings were saved, in order to visualize the action potentials of the ganglion cells detected  and to apply a computational method for the spike sorting. In this way, it was saved the time stamps in which each action potential was fired by its respective cell. This work established a basic experimentation protocol focused to the use of MEA devices in the laboratory for acquisition of extracellular potentials at the Antonio Nariño University – Bogota Headquarters, not only for characterization of action potentials fired by retinal ganglion cells populations, but also for other kind of cells that can be studied employing MEA devices.</p><p> </p>