A new group of nitrogen-fixing Azospirillum sp. bacteria was isolated from the roots of the C 4 -gramineous plant Miscanthus. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and T ; reference strain Ma4 l DSM 13400). Its GMC content is 707 mol %.
A capacidade rizogênica, a brotação e o vigor de estacas caulinares de aceroleira foram avaliados em condições de casa de vegetação na Embrapa Agroindústria Tropical, em Fortaleza-CE, no período de novembro de 2000 a janeiro de 2001, objetivando determinar a parte do ramo e o tamanho mais apropriado para estaquia. O delineamento experimental foi inteiramente casualizado, no esquema fatorial 3x3, correspondendo a três posições no ramo (apical, mediano e basal) e três comprimentos de estacas (10; 15 e 20 cm), com três repetições de 10 estacas por parcela. As estacas, colhidas de plantas com 2,5 anos de idade, foram plantadas em tubetes de 288cm³ contendo uma mistura de casca de arroz carbonizada e vermicomposto, na proporção volumétrica de 2:3, e cultivadas por um período de 60 dias sob condições de nebulização intermitente. Avaliaram-se a percentagem de estacas enraizadas, o peso da matéria seca do sistema radicular e parte aérea, o número de folhas, de gemas sem brotação e as ramificações emitidas por estaca. Os resultados indicaram que estacas caulinares com 10 centímetros de comprimento e colhidas da porção mediana dos ramos são mais apropriadas para a produção de mudas de aceroleira por estaquia, sob condições de casa de vegetação com nebulização intermitente.
Resumo -Este trabalho objetivou avaliar o efeito de uma bactéria diazotrófica isolada de abacaxizeiro (Ananas comosus) sobre o crescimento e o acúmulo de nutrientes de mudas micropropagadas da cultivar Cayenne Champac, crescidas em tubetes com diferentes substratos. Os substratos foram: casca de arroz carbonizada, bagana de carnaúba e vermicomposto; casca de arroz carbonizada, pó de coco e vermicomposto; casca de arroz carbonizada, vermiculita e vermicomposto; casca de arroz carbonizada, bagana de carnaúba e vermiculita; casca de arroz carbonizada e pó de coco; e casca de arroz carbonizada e vermiculita. O isolado de bactéria foi identificado pela similaridade (97% a 98%) de seqüências de DNAr 16S com a espécie Asaia bogorensis. Após a inoculação do isolado bacteriano nas mudas micropropagadas, estas foram transferidas para os substratos dos tubetes. Aos quatro meses de aclimatação, por ocasião de colheita, as mudas estavam aptas para o plantio no campo. A ocorrência de bactéria diazotrófica relacionada a A. bogorensis em abacaxizeiros está sendo apresentada pela primeira vez. O isolado de bactéria diazotrófica AB219 propiciou maior taxa de sobrevivência e, no substrato com casca de arroz carbonizada, vermiculita e vermicomposto, maior acúmulo de massa seca de raízes das plantas. O conteúdo de sódio nas mudas foi superior com a inoculação bacteriana, independentemente do substrato.Termos para indexação: Ananas comosus, Asaia bogorensis, inoculação, micropropagação. Effect of diazotrophic bacteria on pineapple Cayenne Champac plantlets with different substratesAbstract -The objective of this work was to evaluate the effect of a diazotrophic bacteria isolated from pineapple (Ananas comosus) plants on plantlets growth and nutrient accumulation of the cultivar Cayenne Champac grown with different substrates in tubules conditions. Treatments consisted of six substrates: carbonized rice hulls + leave dust from carnauba (Copernicia cerifera) palm tree + vermicompost; carbonized rice hulls + rip coir dust + vermicompost; carbonized rice hulls + vermiculite + vermicompost; carbonized rice hulls + leave dust from carnauba palm tree + vermiculite; carbonized rice hulls + ripe coir dust; and carbonized rice hulls + vermiculite. The bacterium isolate was identified by similarity (97% to 98%) of sequences of 16S rDNA with the species Asaia bogorensis. After the bacterial inoculation, plants were transferred to substrates into tubules conditions. After four months, at harvesting, acclimated plants were ready to be planted in the field. The occurrence of diazotrophic bacteria related to A. bogorensis in pineapple plants is presented here for the first time. The bacterial isolate AB219 promoted higher plant surviving onto tubules conditions, and induced higher root weight with the substrate of carbonized rice hulls, vermiculite and vermicompost. Higher sodium contents were detected in bacterized plants, independently of substrates.
-The objective of this work was to evaluate the growth and the production of banana plants with resistance to black leaf streak disease (BLSD), in comparison with the performance of traditional cultivars susceptible to the disease. Twenty cultivars were planted at the Jaguaribe-Apodi plateau, in Northeastern Brazil: 15 with resistance to BLSD,
The objective of this work was to evaluate the effect of endophytic diazotrophic bacteria Herbaspirillum and Burkholderia on the Fusarium oxysporum f. sp. cubense (Foc) establishment and on the plantlets growth of the 'Maçã' banana (Musa spp., group ABB). Two assays were carried out in a greenhouse at the National Center of Tropical Agroindustry in Fortaleza city, Ceará State (Brazil), using randomized block designs in the factorial arrangements 4×2 and 8×2. On the first trial plantlets were inoculated with the Burkholderia sp. AB202; Herbaspirillum sp., BA227, both of strains and controls bacteria, with and without Foc and cultivated in pots filled with an autoclaved mixture of washed sand and vermiculite (ratio 3:2 v v −1 ), during 4 months. On a second assay, plants were subjected to following conditions: absence and presence of strains AB202, AB213 (Burkholderia spp.), BA227, BA234 (Herbaspirillum-like), AB202 plus BA227, AB213 plus BA234, the mixture of the four bacterial strain, absence and presence of the Foc; and cultivated in pots filled with autoclaved Haplic Arenosol, during 2 months. The plant association with diazotrophs and the Foc was confirmed, and factors interacted significantly on the most probable number of bacteria and the colony forming units of the pathogen on roots and plant rhizomes. The potential of the endophytes on the inhibition of Foc propagate units and on the plant growth promotion was demonstrated. The higher biomass was observed four and 2 months after plant inoculation with AB202 and BA234. Results showed that these endophytes may be used as potential biofertilizer and biocontrol agents.
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