Aim:The therapeutic evaluation of the biological effect of platelet-rich plasma (PRP) used as a surgical adjunct to maintain the inflammatory process and to potentiate tissue healing, make the subject of recent research in regenerative medicine. This study was designed to evaluate the healing activity of PRP by its topical application on the skin experimentally injured in a sheep model.Materials and Methods:The study was conducted on 9 adult and clinically healthy males sheep. PRP was obtained by a protocol of double centrifugation of whole blood from each animal. After sterile skin preparation, full-thickness excisional wounds (20 mm x 20 mm) were created on the back of each animal. The animals were randomly divided into three equal groups of three sheep for each. In Group I, the wounds were treated with PRP, in Group II; wounds were treated with Asiaticoside; in Group III, wounds were treated with saline solution. The different treatments were administered topically every 3 days. Morphometric measurements of the contraction surface of the wounds and histopathological biopsies were carried out at the 3rd, 7th, 14th, 21st, and 28th days of healing.Results:The results of the morphometric data obtained revealed that it was significant differences recorded at the 7th and 14th day of healing in favor for animals of Group I. Semi-quantitative histopathological evaluation showed that PRP reduces inflammation during 3 first days post-surgical and promotes epithelialization in 3 weeks of healing.Conclusion:We concluded that topical administration of PRP obtained by double centrifugation protocol could potentially improve the skin healing process in sheep.

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The aim of the present study was to investigate the influence of the age, the sex and the breed on the diameter, the circumference and the surface area of red blood cells in the dog. Samples were taken from 88 local Sloughi and German shepherd dogs, and the morphometric study was done using OPTIKA TM Vision Pro software. The obtained data indicated that the age and the sex had no influence on the three morphometric parameters of erythrocytes (P>0.05). However, the breed seemed to have a significant effect only on the diameter of erythrocytes, in this case: red blood cells of local Sloughi dogs were bigger than those of the German Shepherd (P<0.001). The breed had no influence on the circumference and surface area of erythrocytes. This study allows us to propose new reference values for the circumference and the surface of erythrocytes in Sloughi and German Shepherd dogs; these both parameters appear most representative as to mark changes in the morphometry of red blood cells.