Aim: The study aimed at evaluating the seroprevalence of H. pylori infection and its associated risk factors in a cross-section of asymptomatic adult population in Niger-Delta, Nigeria. Methodology: 408 apparently healthy volunteers, aged between 18 -87 years were recruited for this study. Blood samples were collected from participants and analyzed for H. pylori antibody (IgG) qualitatively with Combo rapid kits and quantitatively with Accu-Bind ELISA Kits. Results: The overall prevalence of Helicobacter pylori colonization in 408 asymptomatic adults was 52.5% (n = 214) and 48.3% (n = 197) by qualitative (Combo rapid kits) and quantitative (Accu-Bind ELISA Kits) serological test methods respectively. H. pylori infection did not differ statistically between genders (p = 0.962) and among age groups (p = 0.185). In addition, multivariate logistic regression indicated that sex and age were not associated with risk of H. pylori. However, participants from Delta Central were at greater risk (OR = 1.89; p = 0.014) of H. pylori infection compared with those from Delta South, but those from Delta North were not at greater risk of infection compared with those from Delta South (p = 0.476). Conclusion: This study indicated an intermediate seroprevalence of H. pylori among asymptoHow to cite this paper: Omosor, K.I.,