BackgroundThe emergence of Anopheles populations capable of withstanding lethal doses of insecticides has weakened the efficacy of most insecticide based strategies of vector control and, has highlighted the need for further studies on the mechanisms of insecticide resistance and the various factors selecting resistant populations of mosquitoes. This research targeted the analysis of breeding sites and the oviposition behaviour of susceptible and resistant populations of Anopheles in localities of spilled petroleum products. The aim was to establish the possible contribution of oil spillage in the selection of pyrethroid resistance in malaria vectors.MethodsAnopheles breeding sites were identified and the insecticide susceptibility of the Anopheles gambiae populations mapped in 15 localities of South Western Nigeria. The presence of oil particles as well as the turbidity, the dissolved oxygen and the pH of each identified breeding site was recorded. Data were cross-analysed to correlate the habitat types and the insecticide susceptibility status of emerging mosquitoes. The second phase of this study was basically a laboratory model to provide more information on the implication of the spillage of petroleum on the selection of pyrethroid resistance in An. gambiae.ResultsModerate levels of resistance following exposure to permethrin-impregnated papers were recorded with the majority of An. gambiae samples collected in the South Western Nigeria. Data from this study established a link between the constituency of the breeding sites and the resistance status of the emerging Anopheles.ConclusionThis study has revealed the segregational occupation of breeding habitats by pyrethroid resistant and susceptible strains of An. gambiae in south-western Nigeria. Compiled results from field and laboratory research point out clear relationships between oil spillage and pyrethroid resistance in malaria vectors. The identification of this factor of resistance could serve as strong information in the management of insecticide resistance in some West African settings.

This study determined the transition-transversion mutation in the pks gene of Aspergillus section Nigri in order to gain insight into the patterns of nucleotide base substitution and the process of molecular evolution using standard recommended techniques. Results obtained depict frequent occurrence of transition (23 ± 0.96) than transversion (11.37 ± 1.38) (p < 0.05) with C/T being the most frequently observed transitional base substitution and C/A the most frequently occurring transversional base change. The number of single base insertions (56 ± 1.00) were significantly higher than the observed single base deletions (38 ± 2.00) (p < 0.05) while varying degrees of two or more base deletions and insertions were also observed both inside and outside the open reading frame. The maximum likelihood value estimated for the pks gene was calculated to be -9458.80 in 423 positions of the final dataset while the transition-transversion ratio was estimated to be 0.50. The Tajima's neutrality test approaches seven (7) with the nucleotide diversity estimated to be approximately 65%. Evolutionary test depicts positive selection as ratio of non synonymous to synonymous divergence was found to be greater than ratio of the number of non synonymous to synonymous polymorphisms. The proportion of substitution driven by positive selection was calculated to be approximately 96.2%. This research therefore provides an insight into the understanding of pks gene mutation patterns as some of the observed indels resulted in frame shift mutations.

Background: Asymptomatic bacteriuria (ASB) in children is a predisposing factor to symptomatic urinary tract infection (UTI) that may be complicated by blood stream infections if not appropriately treated with resultant mortality or morbidity. The objectives of this study are to determine the prevalence of ASB, and evaluate both biochemical and bacteriological characteristics of urine samples of primary school pupils in Ago-Iwoye, Ijebu North Local Government Area (LGA), Ogun State, Nigeria. Methodology: Three hundred and seventy-two (186 males and 186 females) apparently healthy (asymptomatic) pupils aged 2-16 years from four randomly selected primary schools in the LGA were screened for ASB. Clean catch specimen of midstream urine was collected from each subject. Biochemical analysis of the urine was performed with Combi 10 reagent strip. MacConkey and Cysteine Lactose Electrolyte Deficient (CLED) agar plates were inoculated with calibrated wireloop delivering 0.01 ml of urine for aerobic culture at 37 o C for 24 hours. Identification of significant bacteria on culture plates was done using conventional biochemical tests. Results: The frequency of clear, slightly turbid and turbid urine were 31 (8.3%), 99 (26.6%) and 56 (15.1%) respectively. All analyzed urine samples were alkaline and negative for ketone, glucose and blood, but contained protein in 230 (61.8%), bilirubin in 184 (49.5%), nitrites in 64 (17.2%) and urobilinogen in 14 (3.7%) subjects. The prevalence of significant bacteriuria was 11.8% (44 of 372) with 7.0% in males and 16.7% in females (p = 0.0063). The frequency of bacteria isolated in descending order were Escherichia coli 61.4%, Staphylococcus saprophyticus 61.4%, Staphylococcus aureus 45.5%, Bacillus subtilis 45.5%, Enterococcus faecalis 43.2%, Enterobacter spp 36.4%, Serratia marscencen 31.8%, Klebsiella pneumoniae 22.7%, Proteus mirabilis 22.7% and Pseudomonas aeruginosa 20.5%. Conclusion: This result highlights the presence of significant bacteriuria among apparently healthy pupils in the study area, with higher prevalence in the female pupils. The apparent risk of developing symptomatic UTI with the attendant complications in these pupils should spur preventive education of parents/guardians and the general populace about this entity.

The role of ochratoxigenic moulds in causing several invasive diseases has been documented but little is known about the effects of these ochratoxigenic moulds contaminated feeds in a laboratory control experiment. This study therefore, examined the pathological effects of ochratoxigenic moulds contaminated feed in order to understand their reverberations on different organs. Thirty two albino rats were randomly assigned to four experimental treatments (T) with eight rats per treatment (T1, T2, T3 and T4). T1 and T2 were female and male control rats, respectively that were fed normal feed, while T3 and T4 were male and female experimental rats that were fed with ochratoxigenic moulds contaminated feed for a period of two weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), acid phosphate (ACP), total protein, albumin and histopathological analysis of different organs, namely liver, heart, kidney, stomach, small intestine, lungs, brains and spleens were carried out following standard protocols. Results obtained depicted varying levels of serum concentrations of total protein, albumins, AST, ACP, ALT and ALP between treatments and control groups (p < 0.05). Photomicrograph examinations of the different organs examined reveal degenerative changes in the ultra-structural integrity of all the organs as compared to the control groups except for the spleen where there was no difference between the control and the treatment group. Keywords: Pathology, scanning, ochratoxigenic moulds, reverberations.

Introduction There are lapses in Nigeria’s data ecosystems with the consequences of imprecise and inaccurate data on humanitarian crises limiting accurate interventions. Therefore, we examined the data targeting processes in the humanitarian sector of Northeast Nigeria and the ethical concerns that arise when such data is collected and used to advance understanding and improve humanitarian protection systems. Methods The fieldwork was done in two phases in Maiduguri Borno, North-East Nigeria, between 2021 and 2022. This period was selected because it was the climax of IDP camps in the Northeastern part of the country. Maiduguri was selected for the study because it is the capital of Borno state which is the epicenter of insurgency and internal displacements in Nigeria. Hence, a lot of the most vibrant IDP camps in Nigeria were in Maiduguri for care and security reasons. Fifty in-depth interviews were conducted among the displaced persons across five camps. We also interviewed twenty stakeholders and practitioners working with IDPs to understand Nigeria’s data-based humanitarian contexts of internal displacement. The interviews were tape-recorded and transcribed verbatim by a language expert. The data were coded, and content analyzed to provide context and explore significant operational and ethical issues in data-driven humanitarian protection. Results There are discrepancies in the definition of vulnerability in data gathering, putting into question how targeting is carried out to identify vulnerable people and its implications for exclusion. Different data banks and reliability issues across institutions and actors make room for a multiplicity of data and problematic synergy relative to data and ethics. Inconsistent ethical systems guide data gathering and utilization in IDP camps; for instance, there are ineffective norms of recording and securing informed consent during data gathering. States, partners, and IDP camps confront debilitating capacity gaps and equipment deficits that make updated data gathering, storing, retrieval, and utilization. Paper and digital data storage processes were often used with restricted access to only a few key stakeholders. There is vast data expropriation without standard recourse to justice and beneficence as ethical procedures in the humanitarian data space of northeastern Nigeria as a microcosm of Sub-Saharan African realities. Conclusion There are enormous implications for effective and efficient targeting processes and outcomes, strategic inclusion, and ethical practices in conflict management, humanitarian interventions, and internal displacement in sub-Saharan Africa.