This study was carried out to investigate the effect of equi-protein replacement of soybean meal with groundnut cake on immune status, haematology and serum biochemical parameters of broiler chickens. A total of One hundred and twenty (120) Cobb’s day old chicks were randomly allocated to five (5) dietary treatments of three (3) replicates and eight (8) chicks per replicate in a completely randomized design. The Diet I was the control diet with 100% SBM as the major plant protein source while in other diets, SBM was replaced with 25, 50, 75 and 100% GNC in equi-protein basis and designated as Diets II, III, IV and V respectively. Feed and water were provided ad-libitum throughout the experimental period of 56 days. Newcastle disease (ND) vaccines were administered following a stipulated regime. At the end of the experiment two birds were randomly selected from each replicate and slaughtered. Blood and serum was collected for haematological analysis, serum bioassay and HA/HI tests to determine antibody titres against ND vaccines. All data collected were subjected to one-way analysis of variance using SPSS software package. Results showed that haematological parameters were significantly influenced by the dietary treatments in which birds fed 50% equi-protein replacement of SBM with GNC had better PCV, basophil, MCH and MCHC values compared to that of birds in the other dietary treatments. The antibody titres of experimental birds were not significantly different among treatment groups. The diet with 50% equi-protein replacement of SBM with GNC elicited the highest antibody titres of Log28 and Log210 after the 1st and 2nd ND vaccinations when compared to the other diets. The serum total protein, globulin, albumin, and glucose were significantly influenced by the dietary treatments. The present result indicate that 50% equi-protein replacement of SBM with GNC in diets is not detrimental to health status of broiler chickens.
The many benefits of all parts of neem (Azadirachta indica) tree are well documented. Thus, this study was done to ascertain the effects of sundried neem leaf meal (NLM) on growth performance, shank pigmentation and blood profile of broiler chickens. One hundred and forty four day-old chicks were randomly distributed after 1 week pre-experimental period to four experimental diets which comprised NLM at 0% (control), 5% (5NLM), 10% (10NLM) and 15% (15NLM). A completely randomized design was adopted with 12 birds per replicate and 3 replicates per treatment. Feed and water were given ad-libitum and other management practices were carried out. Initial weight of chicks and final weights at the end of the starter and finisher phases were taken. Feed intake was measured weekly and feed conversion ratio (FCR) calculated. Shank pigmentation was assessed from 3 birds/ replicate at the end of the trial. Blood samples were collected from 3 birds/ replicate at the end of the starter and finisher phases. For the starter phase, final weight, total weight gain and FCR were significantly influenced by NLM inclusion. Chicks fed control and 5NLM diets had similar higher values than others. Blood parameters were not significant except basophil values. At the finisher phase, total weight gain and FCR were not significantly different although feed intake significantly reduced with inclusion of NLM in the diets. Blood parameters were not significant except lymphocyte values. There was a non-significant increase in shank pigmentation with increase in NLM inclusion. It is concluded that NLM inclusion in broiler diets at both phases should not exceed 5% based on growth performance. Neem leaf meal increased yellow colouration of shank and was not harmful to broiler blood parameters.
Abstract-This trial was designed to study the effects of using composite leaf meal produced from five (5)
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