Ethanol production is also known as ethanolic fermentation in which sugars of biomaterials are converted into the ethanol and carbon hydroxide that may called as coproduct of fermentation. In general fermentation is a bio-chemical process where decomposition of biomaterials takes place. During bio-chemical reduction the sugar compounds like sucrose, fructose, glucose and lactose are converted into the ethyl ethanol and CO 2 as by product of the fermentation process. The yield of ethanol greatly depends upon the amount of sugar content, conversion rate (fermentation rate), type of culture and aerobic and anaerobic condition. In this study the one of the agricultural produce i.e. broken rice was taken as the source of sugar for ethanol production because it has considerable lower market value as compare to whole rice. The substrate of broken rice was pre-treated with different method in order to release the sugars. The more the reducing sugar results higher the ethanol production.