This study examined Jordanian university students' knowledge of the citizenship concept while also aiming to reveal whether there were any statistically significant differences in students' knowledge of the citizenship concept due to gender, residence, family income, college, academic level and accumulative grade point average. The study sample comprised 900 B.A level enrolled at six Jordanian universities in the academic year 2012/2013. The research tool was a questionnaire comprising 56 items in four dimensions (identity, loyalty, pluralism, freedom and political participation). Study findings revealed a high level of knowledge of the citizenship concept the total level of the Jordanian university students' in all dimensions was high, despite a medium rating for freedom and political. Results showed statistically significant differences as follows: with regard to the gender variable on the dimensions of identity, pluralism, and freedom and political participation, in favor of males; among rural and urban students on the dimension of loyalty, in favor of rural students; on the dimension of freedom and political participation, in favor of urban students; in favor of high income students on the dimensions of identity, pluralism, freedom and political participation; among students of scientific and humanities colleges on the dimensions of pluralism and freedom and political participation, in favor of humanities colleges; with regard to student academic level on the dimensions of loyalty, pluralism, and freedom and political participation, in favor of the fourth year students. Finally, there were statistically significant differences with regard to the students' accumulative grade point average variable in favor of students with grades of excellent and very good.