No abstract

The present study was planned to investigate the clinicoplathological and histopathological changes on the blood of catfish (Clarias gariepinus) infected with Pseudomonas florescence. A total number of 240 catfish were collected from Ismailia channel and subdivided to 4 groups 60 for each: the control group, infected group, β-glucan group and β-glucan infected group. The blood samples were taken at 1, 3 and 5 weeks for hematological and biochemical examinations. Tissue samples from spleen, liver, kidney and gills were taken for histopathological techniques. The hematological results revealed significant decrease of RBCs count, Hb concentration and PCV% with significant increase of T.L.C, heterophils and lymphocytes of the infected group compared to the control group. The β-glucan groups showed significant increase of RBCs count, Hb and PCV% with significant increase of T.L.C, heterophils, lymphocytes and monocytes. Serum biochemical results showed marked elevation in ALT, AST, urea, creatinine, uric acid and glucose of the infected group. While, βglucan improved the liver function, kidney function tests and glucose before and after the infection compared to the control group. The challenge test revealed a significant lower mortality percentage in the group received β-glucan supplemented diets. Histopathologically, marked degenerative changes and necrosis were evident in the infected group. While, β-glucan groups showed hyperactivity of melenomacrophage centers of haemopiotic organs. It could be concluded that β-glucan acts as a dietary supplement.

The present study was planned to investigate the immunological changes in blood and mucus of catfish Clarias gariepinus infected with Pseudomonas florescence along with trial for control using (βglucan). A total number of 240 catfish were collected from Ismailia channel and subdivided to 4 equal groups: the control group, infected group with virulent strain of P. florescence, β-glucan group and βglucan infected group fed on diet supplemented with β-glucan. The blood and mucus samples were taken at 2 days, 3 and 5 weeks for immunological examinations. There were significant decreases in total protein and albumin of the infected group, while β-glucan and β-glucan infected groups revealed significant increases in total protein and globulin compared to the control group. The serum and mucus immunological studies revealed enhanced immune parameters (lysozyme activity and IgM) in all groups with nonsignificant change in protease level in serum but increased in mucus only compared to the control group. These results concluded that dietary supplementation of β-glucan enhanced skin mucosal and serum humoral defence responses and resistance of catfish against infection with P. florescence. The results could be useful for better understanding the role of skin mucus as a key component of the innate immune system, which could be beneficial in control of fish health.

A total number of 60 Clarias gariepinus fish obtained from Ismailia governorate and its tributaries were collected from three locations. The fish were divided into three main groups, (group A) from El-Teraa, (group B) from El-Berkaa, (group C) from El-Rashah. These locations derived from Mohamed Ali channel which derived from River Nile. The fish and water of control group were obtained from central laboratory for Aquaculture Research, El-Abbassa, Abo-Hamad, Sharqia, Egypt. Water analysis of the examined polluted locations revealed high level of ammonia. Serum biochemical examinations revealed hypoproteinemia, hypoalbuminemia and hypoglobulinemia with increase in serum ALT, AST, total bilirubin, direct bilirubin, indirect bilirubin, glucose, urea, creatinine and serum ammonia level in the three groups compared with control one.