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An investigation of runoff property of pesticides applied in a golf course was conducted by using lysimeters and at actual golf courses. In lysimeter experiment, a remarkable leaching was observed in only 5 pesticides [trichlorfon (DEP), metalaxyl, isoprothiolane, flutolanil and iprodione] with at least 100mm of precipitation.The leaching was scarce in other 12pesticides [bensulide (SAP), isofenphos, fenitrothion (MEP), fenarimol, triflumizole, tetrachlorvinphos (CVMP), pyridaphenthion, oxine-copper, isoxathion, prothiofos, tolclofosmethyl, pendimethalin] with sum of 440mm of precipitation for 51 days. The leaching pattern of the 5 pesticides showed a single broad peak. There was a tendency that pesticides with a high water solubility had a potential to move downward.In putting green experiment, all the 3 pesticides applied (isofenphos, flutolanil and metalaxyl) were detected in leaching water with only 10mm of precipitation.There was no significant difference among the pesticides in the concentrations during experimental period for 41 days. In fairway experiment, all the 4 pesticides (propyzamide, SAP, MEP and iprodione) were detected in the surface water with at least 10-20 mm precipitation.Concentration of pesticides in the surface water from the putting green and fairway experiments was higher than that in the leaching water.Since the present experiments were carried out during the winter period, attention should be paid to the fact that the environmental conditions are different from those in spring to fall when pesticides are usually applied to golf courses.

Metabolic fate of ETU was investigated in the rats administered orally 100 mg/kg of C14-ETU on the twelfth day of gestation. ETU was absorbed readily from the gastrointestinal tract and passed away from the whole body tissues including the fetus rapidly. Only the exception was the thyroid gland and the radio-activity was accumulated in the gland. Most of the administered activity (80.2%,4,5-C14-ETU) was eliminated into the urine in 24 hr and the tissues (including the fetus) levels of radioactivity from 2-C14-ETU reached maximal within 2 hr and fell down to negligible levels by 24 hr. Radiocarbon(s) of 4,5-C14-ETU was expired as radioactive carbon dioxide and was incorporated into the serum and fetal cell constituents (crude protein fraction), but that of 2-C14-ETU was neither expired or incorporated into the cell constituents. From the fetus extract ETU and several radioactive metabolites were detected.