Introduction HLA-haploidentical hematopoietic stem cell transplant (Haplo HSCT) is a potentially curative strategy for patients lacking a HLA matched donor or a suitable unrelated donor. The use of post-transplant Cyclophosphamide (Cy) for GVHD prophylaxis and the particular characteristics of Haplo-HSCT can be linked to a higher risk of hemorrhagic cystitis (HC) and viral infections. In this context, our aim was to analyze HC in both haploidentical and conventional donor transplants in our center. Material and Methods We retrospective analyzed of 237 patients who received an HSCT in our center, between August-2012 and January-2016. We analyze the incidence of HC in the early post-HSCT period (6 months) and its characteristics in those patients receiving Haplo (n=36, 15%) and we compared it with a non-haploidentical donor transplant group from the same period (n=201, 85%). Results Patients' characteristics are summarized in table 1. Both groups are balanced, except for diagnosis due to the high proportion of Hodgkin lymphoma in Haplo group, conditioning regimen, and GVHD grade III-IV with a lower incidence in Haplo. Conditioning regimen in Haplo consisted of Fludarabine (30mg/m2x or 50mg/kg x 4days in RIC or MA regimen) and Busulfan (3.2 mg/kg x 2 in RIC or 3 days in MA). Post-HSCT Cy was used for GVHD prophylaxis in 94%. Median of days to reach more than 500x10^9 granulocytes and more than 20x10^9 platelets in Haplo group were 18 (13-30) and 23 (0-103) respectively. Hemorrhagic cystitis: Incidence of HC was 33% vs. 13% in conventional HSCT (p=0.03). Infectious etiology was documented in 84% of the Haplo recipients and 89% in conventional HSCT (p=0.87). In Haplo, HC was caused by BK/JC polyomavirus in 67% and adenovirus in 17%. Among conventional HSCT recipients, BK/JC was documented in 75% and adenovirus in 14% of HC. Viral infection was diagnosed by positive PCR in urine. HC appeared at a median of 65 days post-HSCT (5-557), without differences between Haplo and non-Haplo: 61 (5-423) vs. 67 (8-557). Treatment of HC consisted of hyper-hydration and spasmolytic drugs in 19 patients. Intravesical cidofovir was used in 7 and intravenous cidofovir was needed in 2 cases. Receiving an Haplo HSCT was associated with a higher risk of developing HC [OR 3.5 (95% CI 1.56-7.91); p=0.002]. We did not observed association between HC and conditioning regimen (p=0.18), receiving ATG (p=0.45) or presence of acute GVHD grade I-II (p=0.75) or grade III-IV (p=0.46). Haplo donor was the only variable with significant impact in multivariate analysis. Conclusions Incidence of HC is higher in Haplo HSCT, being viral infections and not Cy toxicity, the main cause (polyomavirus BK/JC as the first cause). Table 1 Table 1. Disclosures Mateos: Janssen, Celgene, Amgen, Takeda, BMS: Honoraria.

NPM1+ AML after Essential Thrombocythemia (ET) or Myeloproliferative neoplasms is extremely rare. Only 2 cases have been previously reported after Primary Myelofibrosis. Given the extremely poor prognosis of the Blastic phase of Myeloproliferative neoplasms (MPN-BP), the only curative treatment of these patients is allogeneic stem cell transplantation (allo-SCT) after achieving Complete Response (CR). De novo NPM1+/FLT3- AML is considered a good prognosis entity in which allo-SCT is not contemplated as the first option. A 41 year old diagnosed of ET JAK2 V617F+ 4 years before the diagnosis of AML with normal karyotype and NPM1+/FLT3- was treated with conventional AML induction with Cytarabine and Idarubicine and 3 cycles of high dose Cytarabine. At the diagnosis of AML other 2 mutations were noted: EZH2 and IKZ1. After treatment of AML, NPM1+ clone disappeared, and JAK2 V617F clone reappeared. We opted to treat NPM1+ AML as a de novo AML and we decided to follow up during 2 years without allo-SCT. The patient remains in complete response with NPM1 minimal residual disease negative during the follow up. This case exemplifies the nature of NPM1+ AML secondary to MPD as an extremely sensitive disease to induction therapy plus high dose cytarabine and makes that these type of patients perhaps could be managed without the use of allo-SCT.

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Tritium is one of the required elements in future fusion reactors. This hydrogen isotope has to be continuously produced in order to supply the fusion reactor. Tritium can be obtained from the fussion reaction of lithium 6. Because of that, future fusion reactors are designed to have a tritium breeding module surrounding the reactor. One of the breeding blanket that has been proposed is based on lead-lithium eutectic melt [1]. Nevertheless, lithium monitoring in the breeding blanket is of great importance for the performance of the reactor. A deviation from the eutectic composition can cause a substantial change on the tritium production. Therefore, in order to control the lithium dosage needed and keep the adequate composition in the melt, on-line lithium measurements will be required. For this purpose, lithium probes must be designed to withstand the high temperatures of the breeding modules (~400 ºC) and the chemical reactivity of lithium. Finally, the constructed sensors need to be tested at different lithium concentrations in order to determine its viability and range of application. Sensors based on solid state electrolytes have several advantages: stable compounds which can withstand the harsh chemical environment of the melts, its ionic conductivity tends to increase frequently with the temperature [2] and the output signal (cell potential or electrochemical current) is easy to measure. Lithium conducting electrolytes for molten metals are under development at the Electrochemical Methods Laboratory at Institut Quimic de Sarria (IQS), Barcelona. Its qualification and performance are being tested. Li-probes for molten metals will be based on the use of ceramic type lithium conducting solid-state electrolytes. In the present work, lithium conducting solid electrolytes Li6BaLa2Ta2O12 [3] and Li6La3Ta1.5Y0.5O12 [4] were synthesized. The crystallographic phases were characterized with XRD, its surface microstructure with SEM and its lithium content was determined. The resulting ceramics were shaped in a disc form. The potentiometric probe consisted in the union of the sintered ceramic disc with an alumina tube using a glass sealant. The design of the sensor involved a two electrode system that permitted to measure the potential difference between a working and a reference electrode. The reference electrode was stablished as a lithium alloy with a fixed and known composition which was placed inside the alumina tube. The working electrode was the alloy where the assembly was submerged. A molybdenum wire was used to allow the electron flow from the electrodes to the measuring system. The sensors response was measured potentiometrically in two different lithium alloys Sn/Li and Pb/Li. The experiments that were performed for each lithium alloy consisted on the measurement of the potential difference between the working and the reference electrode. In each experiment, the lithium concentration was increased in the working electrode while the lithium content in the reference electrode was kept constant. The lithium concentration in the melts (working electrode) covered a range from 3at% up to 30at%. The measurements were performed at three different temperatures: 400 ºC, 500 ºC and 600 ºC. Correlation curves between the measured potential and the lithium composition were evaluated. The slopes of the obtained correlations were in good agreement with the slopes of the Nernst equation. References [1] F. De Schutter, J. Dekeyser, J. Luyten, H. Tas, Electrochemical sensors for oxygen and lithium detection in Pb17Li, Fusion Eng. Des. 14 (1991) 241–248. https://doi.org/10.1016/0920-3796(91)90008-E. [2] V. Thangadurai, S. Narayanan, D. Pinzaru, Garnet-type solid-state fast Li ion conductors for Li batteries: critical review, Chem. Soc. Rev. 43 (2014) 4714. https://doi.org/10.1039/c4cs00020j. [3] M. Nel-lo, S. Colominas, J. Abellà, Lithium conducting ceramics for future electrochemical sensors in molten metals, Fusion Eng. Des. (2019). https://doi.org/10.1016/j.fusengdes.2019.02.043. [4] A.K. Baral, S. Narayanan, F. Ramezanipour, V. Thangadurai, Evaluation of fundamental transport properties of Li-excess garnet-type Li5+2xLa3Ta2-xYxO12 (x = 0.25, 0.5 and 0.75) electrolytes using AC impedance and dielectric spectroscopy, Phys. Chem. Chem. Phys. 16 (2014) 11356–11365. https://doi.org/10.1039/c4cp00418c.

INTRODUCTION: Endoscopic and transbrochial ultrasound-guided fine needle aspiration techniques facilitate the sample acquisition process from the deep intrathoracic or intra-abdominal lymph nodes and masses, for cytological analysis, without requiring surgical procedures. These techniques minimize patient's discomfort, are cost-saving and can be applicable as the first diagnostic approach. Accurate analysis of the aspirate material is the key to achieve a correct diagnosis and to conduct further management. The aim of our study is to determine the utility of multiparametric flow cytometry (FC) in comparison to that of cytomorphology (CM) in analyzing samples obtained by endoscopic techniques as screening for hematological malignancies. METHODS: We retrospectively analyzed the results of 166 samples routinely submitted to hematology FC laboratory, obtained by transluminal puncture of adenopathies or masses (124 transbronchial and 42 endoscopic fine needle aspirations) for the screening of hematological neoplasia. All the samples were processed within the first four hours after obtainment. Eight-color, well-standardized panel was used for the screening of lymphoproliferative syndrome (B screening: Kappa-FITC/ Lambda-PE / CD5-PerCP-Cy5.5/ CD19-PE-Cy7/ CD10-APC/ CD38-APCH7/ CD20-V450 /CD45-OC515; and T screening CD2-FITC/ CD5-PE / CD8-PerCP-Cy5.5/ CD7-PE-Cy7/ CD56-APC/ CD4-APCH7/ CD3-V450 /CD45-OC515). At least 1x 10(6) events were acquired by FACSCanto II (BD Biosciences, San Jose, USA). The data was analyzed with the Infinicyt software (Cytognos SL, Spain). Meanwhile, cytomorphology analysis was performed independently by the Pathology department on samples obtained from the same procedure at the same time, with additional immunochemical staining depending on the findings. Finally, results of both techniques were compared to each other and also with the final diagnosis based on the availability of a diagnostic biopsy and the information extracted from clinical history. Statistical analyses were performed using SPSS v20.0 (SPSS Inc., IL USA). RESULTS Comparing the number of evaluable samples using both techniques, FC was more reliable, since 90% (150/166) of the total samples could be evaluated by FC, and only 74% (123/166) were considered evaluable by CM (due to necrosis or hemorrhagic cellularity). Using FC hematological neoplasms were identified in 13% of the total series (22/166): 18 Non Hodgkin lymphoma (NHL) and 4 Multiple Myeloma (MM). In most of the samples (71%; 119/166), the FC approach for screening of lymphoproliferative disorders was negative, and in 9 cases (5%) this approach suggested the presence of a non-hematological neoplasia. Considering CM, although this technique was as useful as FC for the identification of plasma cell dyscrasia identification (4 MM cases detected), it was less useful for NHL diagnosis, detecting only 33% (6/18) of the total number of NHL cases that had been detected by FC. However, CM was more useful for the identification of Hodgkin's Lymphoma (3%; 5/166) and non-hematological neoplasms (14% of carcinomas; 24/166), being the rest of the evaluable samples classified as inflammatory yield (50%; 84/166). In order to reach the definitive final diagnosis, in 66 cases (40%; 66/166) another procedure involving surgery (n= 55) or needle biopsy (n=11) was performed. Eleven new NHL cases were identified, and 8 of these 11 cases were considered as not evaluable for the FC analysis performed in the previous sample, due to low cell viability. In addition, 5 new HL and 10 new carcinoma cases were identified, not diagnosed in previous FNA Cytology. CONCLUSION: Our study of showed that multiparametric FC is more sensitive than CM to diagnose of NHL, while CM is more useful to diagnose solid neoplasms in the analysis of the samples obtained from endoscopic and transbrochial ultrasound-guided FNA technique. Therefore, both techniques should be considered as complementary for the evaluation of these deep thoracic and abdominal nodes or masses. In addition, around 40% of cases require a new biopsy to reach definitive diagnosis. Disclosures Martín: Sevier: Honoraria, Membership on an entity's Board of Directors or advisory committees; Janssen: Honoraria; Gilead: Membership on an entity's Board of Directors or advisory committees; Celgene: Honoraria, Membership on an entity's Board of Directors or advisory committees. Del Cañizo:Celgene: Membership on an entity's Board of Directors or advisory committees, Research Funding; Jansen-Cilag: Membership on an entity's Board of Directors or advisory committees, Research Funding; Arry: Membership on an entity's Board of Directors or advisory committees, Research Funding; Novartis: Membership on an entity's Board of Directors or advisory committees, Research Funding.