Single-cell RNAseq is a powerful tool for the dissection of cell populations. Multiple dimension reduction (DR) tools are available to project cells on to 3-dimensional space that allow one to visualise the heterogeneity within the assayed population, often forming complex cellular maps. Thus far, visualisation methods for 3D embedded maps are poor, and the lack of intuitive point/cell selection often hinders a rapid exploration of finer details contained in the data. Moreover, directly comparing the output from several DR methods is not possible. Here we present CellexalVR (www.cellexalvr.med.lu.se), a feature-rich, fully interactive virtual reality environment for the visualisation and analysis of single-cell RNAseq experiments that allows researchers to intuitively and collaboratively gain an understanding of their data.Single-cell RNAseq (scRNAseq) is a routinely used method to explore the heterogeneity of

HighlightsSingle-cell experiments are often visualized when embedded into three dimensions CellexalVR is a virtual reality environment to visualize all data simultaneously Teams can analyze singlecell experiments together in VR regardless of location Legetth et al., iScience --, 103251 --, 2021 ª 2021 The Author(s).