A new species of Euathlus Ausserer, 1875 (Araneae: Theraphosidae: Theraphosinae), Euathlus vanessae sp. nov. is described and diagnosed. The genus is known from Chile and Argentina, and E. vanessae sp. nov. represents the first record for the genus in Peru, being the new northernmost limit of its known distribution. Distribution and natural history data are provided. Additionally, the conservation status of the new species is discussed and it is proposed as Endangered (EN) according to IUCN criteria.

A new species of Bistriopelma Kaderka 2015, Bistriopelma fabianae sp. nov., from the Arequipa region in southern Peru is described, diagnosed, and illustrated. Specimens were found in a Queñua forest (Polylepis rugulosa Bitter) spread out on the slopes of Pichu Pichu volcano in the buffer zone of Salinas and Aguada Blanca National Reserve. Biological and ecological data of B. fabianae sp. nov. are provided as well as notes on B. kiwicha and B. peyoi, two new species recently described from Apurimac and Cusco regions. Publicación registrada en Zoobank/ZooBank article registered:LSIDurn:lsid:zoobank.org:pub:BB9DF4D9-6684-46AF-9D15-71FEC64CD032Acto nomenclatural/nomenclatural act:Bistriopelma fabianae Quispe-Colca and Kaderka, 2020LSIDurn:lsid:zoobank.org:act:B63485CD-C00D-4E93-AF02-914AD9A566DA

Negayan tarapaca Lopardo, 2005 is currently known from northern Chile. Herein, we report the first record from Peru of this species, which we collected in a Polylepis rugulosa forest in the department of Arequipa.