The objective of this study was to evaluate total antioxidant capacity (TAC), total phenolic content (TPH), and the identification of anthocyanidin and polyphenolic compounds in 13 colored potatoes collected from the Andean region of Bolivia, and understand how the chemical composition correlated with the botanical classification and molecular characterization of genes, ans (anthocyanidin synthase) and stan1 (Solanum tuberosum anthocyanidin synthase), associated with the synthesis of anthocyanidins. The results show the existence of a limited correlation between botanical classification, based on morphological identification and polyphenol composition. No association between genetic grouping of the ans and stan genes and botanical classification was found. However, it was possible to identify a correlation between the ans gene clades and the levels of anthocyanidins as well as of other polyphenols. Thus, this result confirms the concept that potato color can be used in the search for high polyphenol potato cultivars.
I S S N 2348-6201Vol u m e 6 N u m b e r 2 J o u r n a l o f A d v a n c e s i n B i o t e c h n o l o g y 8 4 8 | P a g e S e p t e m b e r 2 0 1 6 w w w . c i r w o r l d . c o m mariateresa.alvarezaliaga@gmail.com ABSTRACTBiofilm is regarded as universal forms of microorganism life in aquatic and industrial wastewater systems as well as in a large number of environments and medical devices relevant for public health, where the exact mechanisms by which biofilm-associated microorganisms elicit infection diseases are still poorly understood.Candida biofilm formation is regulated by different mechanisms where adhesins play a clue role in the yeast attachment to certain surfaces. These adhesins are encoding by ALS3, HWP1 and EAP1 genes among others and they are also considered as Candida virulence factors.Methodologies use to study biofilm productions are intended to verify the biofilm composition, formation steps, tridimensional structure and might facilitate the monitoring of biofilm regarding, antibiotic resistance, degradations, inhibitors, enhanciement biofilm formation and other features.Here, FISH expression a modified method to detect gene expression in situ was used in order to detect ALS3, HWP1 and EAP1 in C. albicans and C. maltosa biofilms, constituting a useful tool to monitor biofilm formations. In this regard, ALS3 expression was identified in C. albicans and C. maltosa biofilms.
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